Anti-alpha 2a Adrenergic Receptor抗体(ab85570)

概述

  • 产品名称Anti-alpha 2a Adrenergic Receptor抗体
    参阅全部 alpha 2a Adrenergic Receptor 一抗
  • 描述
    兔多克隆抗体to alpha 2a Adrenergic Receptor
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human alpha 2a Adrenergic Receptor.

  • 阳性对照
    • This antibody gave a positive signal in Human small intestine and Human pancreas tissue lysates.

性能

应用

Our Abpromise guarantee covers the use of ab85570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 49 kDa).

靶标

  • 功能Alpha-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins. The rank order of potency for agonists of this receptor is oxymetazoline > clonidine > epinephrine > norepinephrine > phenylephrine > dopamine > p-synephrine > p-tyramine > serotonin = p-octopamine. For antagonists, the rank order is yohimbine > phentolamine = mianserine > chlorpromazine = spiperone = prazosin > propanolol > alprenolol = pindolol.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA2A sub-subfamily.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADA2A_HUMAN antibody
    • ADRA2 antibody
    • ADRA2A antibody
    • ADRA2R antibody
    • ADRAR antibody
    • Adrenergic alpha 2A receptor antibody
    • Alpha 2 adrenergic receptor subtype C10 antibody
    • Alpha 2A adrenergic receptor antibody
    • Alpha 2AAR subtype C10 antibody
    • Alpha-2 adrenergic receptor subtype C10 antibody
    • alpha-2-adrenergic receptor, platelet type antibody
    • Alpha-2A adrenergic receptor antibody
    • Alpha-2A adrenoceptor antibody
    • Alpha-2A adrenoreceptor antibody
    • Alpha-2AAR antibody
    • ALPHA2AAR antibody
    • OTTHUMP00000058849 antibody
    • ZNF32 antibody
    see all

Anti-alpha 2a Adrenergic Receptor antibody 图像

  • All lanes : Anti-alpha 2a Adrenergic Receptor antibody (ab85570) at 1 µg/ml

    Lane 1 : Human pancreas tissue lysate - total protein (ab29816)
    Lane 2 : Human small intestine tissue lysate - total protein (ab29276)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 49 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes
  • ICC/IF image of ab85570 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85570, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293 and HepG2 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
  • IHC image of alpha 2a Adrenergic Receptor staining in human Pancreas FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85570, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

Anti-alpha 2a Adrenergic Receptor antibody (ab85570)参考文献

ab85570 has not yet been referenced specifically in any publications.

Product Wall

The blocking peptide for ab85570 is ab102061.

For ab45871, ab54291 can be used.

BLAST alignment of the immunogen sequence for ab65833, anti-alpha 2a Adrenergic Receptor, with your protein of interest returned a result of 81% identity (13/16).



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