Anti-alpha 2a Adrenergic Receptor抗体(ab85570)
Key features and details
- Rabbit polyclonal to alpha 2a Adrenergic Receptor
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-alpha 2a Adrenergic Receptor抗体 -
描述
兔多克隆抗体to alpha 2a Adrenergic Receptor -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details
不适用于: ICC/IF -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Pig -
免疫原
Synthetic peptide corresponding to Human alpha 2a Adrenergic Receptor aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab102061) -
阳性对照
- Recombinant human alpha 2a Adrenergic Receptor protein (ab54291) can be used as a positive control in WB. This antibody gave a positive signal in Human pancreas tissue lysates.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab85570于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
Use a concentration of 1 µg/ml.
|
|
WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 49 kDa).
|
说明 |
---|
IHC-P
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 49 kDa). |
靶标
-
功能
Alpha-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins. The rank order of potency for agonists of this receptor is oxymetazoline > clonidine > epinephrine > norepinephrine > phenylephrine > dopamine > p-synephrine > p-tyramine > serotonin = p-octopamine. For antagonists, the rank order is yohimbine > phentolamine = mianserine > chlorpromazine = spiperone = prazosin > propanolol > alprenolol = pindolol. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA2A sub-subfamily. -
细胞定位
Cell membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 282135 Cow
- Entrez Gene: 150 Human
- Entrez Gene: 11551 Mouse
- Entrez Gene: 399501 Pig
- Entrez Gene: 25083 Rat
- Omim: 104210 Human
- SwissProt: Q28838 Cow
- SwissProt: P08913 Human
see all -
别名
- ADA2A_HUMAN antibody
- ADRA2 antibody
- ADRA2A antibody
see all
图片
-
Anti-alpha 2a Adrenergic Receptor antibody (ab85570) at 2 µg/ml + Human Pancreas at 20 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 49 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesBlocking buffer: 2% BSA
Gel type: MOPS
-
IHC image of alpha 2a Adrenergic Receptor staining in human Pancreas FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85570, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (6)
ab85570 被引用在 6 文献中.
- Hu Y et al. Intrathecal Injection of Ropivacaine Reduces Cervical Resistance in Late-Pregnant Rats. Drug Des Devel Ther 16:1183-1189 (2022). PubMed: 35502424
- Liu X et al. Distribution and relative expression of vasoactive receptors on arteries. Sci Rep 10:15383 (2020). PubMed: 32958803
- Wang W et al. a2A-Adrenergic Receptor Inhibits the Progression of Cervical Cancer Through Blocking PI3K/AKT/mTOR Pathway. Onco Targets Ther 13:10535-10546 (2020). PubMed: 33116632
- Yu Z et al. Single-Cell Transcriptomic Map of the Human and Mouse Bladders. J Am Soc Nephrol 30:2159-2176 (2019). PubMed: 31462402
- Weng X et al. Reduced mitochondrial response sensitivity is involved in the anti-apoptotic effect of dexmedetomidine pretreatment in cardiomyocytes. Int J Mol Med 41:2328-2338 (2018). PubMed: 29328437
- Reed DE et al. Stress increases descending inhibition in mouse and human colon. Neurogastroenterol Motil 28:569-80 (2016). PubMed: 26744175