概述
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产品名称Anti-alpha 2 Macroglobulin抗体[EPR4432]
参阅全部 alpha 2 Macroglobulin 一抗 -
描述兔单克隆抗体[EPR4432] to alpha 2 Macroglobulin
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经测试应用适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC or IP -
种属反应性与反应: Human
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免疫原
Synthetic peptide corresponding to residues in Human alpha 2 Macroglobulin.
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阳性对照
- Human plasma lysate; Human liver tissue
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常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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纯度Tissue culture supernatant
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克隆单克隆
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克隆编号EPR4432
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同种型IgG
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研究领域
应用
Our Abpromise guarantee covers the use of ab109422 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 163 kDa. | |
| IHC-P | 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
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组织特异性Secreted in plasma.
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序列相似性Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
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发展阶段Contrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.
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细胞定位Secreted.
- Information by UniProt
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数据库链接
- Entrez Gene: 2 Human
- Omim: 103950 Human
- SwissProt: P01023 Human
- Unigene: 212838 Human
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别名
- A2m antibody
- A2MG_HUMAN antibody
- Alpha 2 M antibody
see all
Anti-alpha 2 Macroglobulin antibody [EPR4432] 图像
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![Western blot - alpha 2 Macroglobulin antibody [EPR4432] (ab109422)](http://www.abcam.cn/ps/datasheet/images/109/ab109422/alpha-2-Macroglobulin-Primary-antibodies-ab109422-1.jpg)
Western blot - alpha 2 Macroglobulin antibody [EPR4432] (ab109422)Anti-alpha 2 Macroglobulin antibody [EPR4432] (ab109422) at 1/1000 dilution + Human plasma lysate at 10 µg
Predicted band size : 163 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - alpha 2 Macroglobulin antibody [EPR4432] (ab109422)](http://www.abcam.cn/ps/datasheet/images/109/ab109422/alpha-2-Macroglobulin-Primary-antibodies-ab109422-2.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - alpha 2 Macroglobulin antibody [EPR4432] (ab109422)ab109422, at 1/500 dilution, staining alpha 2 Macroglobulin in paraffin-embedded Human liver by Immunohistochemistry.
Anti-alpha 2 Macroglobulin antibody [EPR4432] (ab109422)参考文献
ab109422 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"