Anti-alpha 2 Macroglobulin抗体(ab84176)


  • 产品名称Anti-alpha 2 Macroglobulin抗体
    参阅全部 alpha 2 Macroglobulin 一抗
  • 描述
    兔多克隆抗体to alpha 2 Macroglobulin
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1450 to the C-terminus of Human alpha 2 Macroglobulin.

    (Peptide available as ab93750.)

  • 阳性对照
    • This antibody gave a positive signal in both human plasma total protein and human placenta tissue lysates.



Our Abpromise guarantee covers the use of ab84176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 185 kDa (predicted molecular weight: 163 kDa).
ICC/IF Use a concentration of 1 µg/ml.


  • 功能Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
  • 组织特异性Secreted in plasma.
  • 序列相似性Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
  • 发展阶段Contrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A2m antibody
    • A2MG_HUMAN antibody
    • Alpha 2 M antibody
    • Alpha 2M antibody
    • Alpha-2-M antibody
    • Alpha-2-macroglobulin antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 antibody
    • CPAMD5 antibody
    • DKFZp779B086 antibody
    • FWP007 antibody
    • S863 7 antibody
    see all

Anti-alpha 2 Macroglobulin antibody 图像

  • All lanes : Anti-alpha 2 Macroglobulin antibody (ab84176) at 1 µg/ml

    Lane 1 : Human Plasma Total Protein Lysate
    Lane 2 : Human placenta tissue lysate - total protein (ab29745)

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 163 kDa
    Observed band size : 185 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 15 minutes
  • ICC/IF image of ab84176 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84176, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 cells at 1µg/ml, and in 100% methanol fixed (5 min) MCF7 cells at 5µg/ml.

Anti-alpha 2 Macroglobulin antibody (ab84176)参考文献

ab84176 has not yet been referenced specifically in any publications.

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Application Western blot
Sample Human Tissue lysate - whole (human heart left ventricular)
Loading amount 40 µg
Specification human heart left ventricular
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Dawn Bowles

Verified customer

提交于 Jan 10 2013