Anti-alpha 2 Macroglobulin抗体(ab58703)
Key features and details
- Rabbit polyclonal to alpha 2 Macroglobulin
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-alpha 2 Macroglobulin抗体
参阅全部 alpha 2 Macroglobulin 一抗 -
描述
兔多克隆抗体to alpha 2 Macroglobulin -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Synthetic peptide corresponding to C terminal residues of human alpha 2 Macroglobulin.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified by antigen specific affinity chromatography. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab58703于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (2) | |
IHC-P | (2) |
说明 |
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ICC: 1/500.
IHC-P: Use at a concentration of 2 µg/ml.
WB: Use at an assay dependent concentration. Predicted molecular weight: 163 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. -
组织特异性
Secreted in plasma. -
序列相似性
Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family. -
发展阶段
Contrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 2 Human
- Entrez Gene: 232345 Mouse
- Omim: 103950 Human
- SwissProt: P01023 Human
- SwissProt: Q61838 Mouse
- Unigene: 212838 Human
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别名
- A2m antibody
- A2MG_HUMAN antibody
- Alpha 2 M antibody
see all
图片
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All lanes : Anti-alpha 2 Macroglobulin antibody (ab58703) at 1/1000 dilution
Lanes 1-3 : lysates prepared from rat spinal cord tissues (control group)
Lanes 4-6 : lysates prepared from rat spinal cord tissues (encephalomyelitis group)
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG coupled to horseradish peroxidase
Predicted band size: 163 kDa
Observed band size: 163 kDa
Additional bands at: 60 kDa (possible cleavage fragment), 80 kDa (possible cleavage fragment)
Three independent control and encephalomyelitis samples were used for Western blotting analysis. Samples were first resolved on a 12.5% SDS-PAGE gel and then transferred to a nitrocellulose membrane. The membrane was rinsed with PBS and the non specific binding sites were blocked in a solution of 5% non-fat milk in PBST (0.05% Tween 20 in PBS) for 1 hour at room temperature, followed by three washes in PBST for 10 minutes each. The membrane was first incubated with ab58703 at a 1/1,000 dilution overnight and then washed in PBST buffer as described above. The immunocomplexes were visualized by Western Lightning® Western Blot Chemiluminescence Reagent Plus, using a goat anti-rabbit IgG coupled to horseradish peroxidase as the secondary antibody. -
Ab58703 staining alpha 2 Macroglobulin in human liver.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
Anti-alpha 2 Macroglobulin antibody (ab58703) at 1 µg/ml + Native Human alpha 2 Macroglobulin protein (ab77935) at 0.1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 163 kDa
Exposure time: 3 minutes
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab58703 被引用在 10 文献中.
- Liu Y et al. CD9, a potential leukemia stem cell marker, regulates drug resistance and leukemia development in acute myeloid leukemia. Stem Cell Res Ther 12:86 (2021). PubMed: 33494824
- Patel MR & Weaver AM Astrocyte-derived small extracellular vesicles promote synapse formation via fibulin-2-mediated TGF-β signaling. Cell Rep 34:108829 (2021). PubMed: 33691102
- Actis Dato V & Chiabrando GA Activated Alpha-2 Macroglobulin Improves Insulin Response via LRP1 in Lipid-Loaded HL-1 Cardiomyocytes. Int J Mol Sci 22:N/A (2021). PubMed: 34203120
- Li Y et al. Potential Serum Biomarkers for Postoperative Neurocognitive Disorders Based on Proteomic Analysis of Cognitive-Related Brain Regions. Front Aging Neurosci 13:741263 (2021). PubMed: 34658843
- Liu X et al. MiR-146b accelerates osteoarthritis progression by targeting alpha-2-macroglobulin. Aging (Albany NY) 11:6014-6028 (2019). PubMed: 31422941
- Wang H et al. HuoXueJieDu Formula Alleviates Diabetic Retinopathy in Rats by Inhibiting SOCS3-STAT3 and TIMP1-A2M Pathways. Int J Genomics 2017:4832125 (2017). PubMed: 29318137
- Constantinescu P et al. Amorphous protein aggregates stimulate plasminogen activation, leading to release of cytotoxic fragments that are clients for extracellular chaperones. J Biol Chem 292:14425-14437 (2017). PubMed: 28710283
- Tomazic PV et al. Nasal mucus proteomic changes reflect altered immune responses and epithelial permeability in patients with allergic rhinitis. J Allergy Clin Immunol N/A:N/A (2013). WB ; Human . PubMed: 24290289
- Jain MR et al. Altered proteolytic events in experimental autoimmune encephalomyelitis discovered by iTRAQ shotgun proteomics analysis of spinal cord. Proteome Sci 7:25 (2009). WB ; Rat . PubMed: 19607715
- Fonseca BM et al. Anandamide-induced cell death: dual effects in primary rat decidual cell cultures. Placenta 30:686-92 (2009). WB, ICC ; Rat . PubMed: 19560819