Anti-alpha 2 Macroglobulin抗体(ab52651)

概述

  • 产品名称
    Anti-alpha 2 Macroglobulin抗体
    参阅全部 alpha 2 Macroglobulin 一抗
  • 描述
    小鼠多克隆抗体to alpha 2 Macroglobulin
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat
  • 免疫原

    Synthetic peptide: KAITYLNTGY QRQLNYKHRD GSYSTFGDKP GRSHANTWLT , corresponding to amino acids 1003/1042 of Mouse alpha 2 Macroglobulin

  • 常规说明


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 50% Glycerol, Whole serum
  • 纯度
    Whole antiserum
  • Primary antibody说明
    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab52651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution. Predicted molecular weight: 163 kDa. This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

靶标

  • 功能
    Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
  • 组织特异性
    Secreted in plasma.
  • 序列相似性
    Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
  • 发展阶段
    Contrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A2m antibody
    • A2MG_HUMAN antibody
    • Alpha 2 M antibody
    • Alpha 2M antibody
    • Alpha-2-M antibody
    • Alpha-2-macroglobulin antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 antibody
    • CPAMD5 antibody
    • DKFZp779B086 antibody
    • FWP007 antibody
    • S863 7 antibody
    see all

Anti-alpha 2 Macroglobulin antibody 图像

  • All lanes : Anti-alpha 2 Macroglobulin antibody (ab52651) at 1/1000 dilution

    Lane 1 : (Left) a total protein extract from E coli with 50ng to 100 ng of a tag fusion protein of an irrelevant antigen
    Lane 2 : (Right) a total protein extract from E coli with 50ng to 500ng of the antigen (tag-antigen fusion protein)

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 5000

    Predicted band size : 163 kDa

实验方案

Anti-alpha 2 Macroglobulin antibody (ab52651)参考文献

This product has been referenced in:
  • de Castro Brás LE  et al. Plasma fractionation enriches post-myocardial infarction samples prior to proteomics analysis. Int J Proteomics 2012:397103 (2012). Read more (PubMed: 22778955) »

See 1 Publication for this product

Product Wall

Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Username

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提交于 Apr 18 2016

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