概述

  • 产品名称Anti-alpha 2 Macroglobulin抗体
    参阅全部 alpha 2 Macroglobulin 一抗
  • 描述
    兔多克隆抗体to alpha 2 Macroglobulin
  • 特异性This antibody is specific for alpha 2 Macroglobulin.
  • 经测试应用适用于: RIA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length native alpha 2 Macroglobulin protein (purified from plasma) (Human).

性能

应用

Our Abpromise guarantee covers the use of ab48555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
RIA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 163 kDa.

靶标

  • 功能Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
  • 组织特异性Secreted in plasma.
  • 序列相似性Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
  • 发展阶段Contrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A2m antibody
    • A2MG_HUMAN antibody
    • Alpha 2 M antibody
    • Alpha 2M antibody
    • Alpha-2-M antibody
    • Alpha-2-macroglobulin antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 antibody
    • CPAMD5 antibody
    • DKFZp779B086 antibody
    • FWP007 antibody
    • S863 7 antibody
    see all

Anti-alpha 2 Macroglobulin antibody 图像

  • Anti-alpha 2 Macroglobulin antibody (ab48555) at 1 µg/ml + Human placenta tissue lysate - total protein (ab29745) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 163 kDa
    Observed band size : 175 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa,60 kDa,80 kDa. We are unsure as to the identity of these extra bands.

Anti-alpha 2 Macroglobulin antibody (ab48555)参考文献

This product has been referenced in:
  • Liu Z  et al. Tandem 18O stable isotope labeling for quantification of N-glycoproteome. J Proteome Res 9:227-36 (2010). WB ; Human . Read more (PubMed: 19921957) »
  • Insenser M  et al. Proteomic analysis of plasma in the polycystic ovary syndrome identifies novel markers involved in iron metabolism, acute-phase response, and inflammation. J Clin Endocrinol Metab 95:3863-70 (2010). WB ; Human . Read more (PubMed: 20484478) »

See all 2 Publications for this product

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