Anti-alpha 2 Glycine Receptor抗体(ab97628)

概述

  • 产品名称Anti-alpha 2 Glycine Receptor抗体
    参阅全部 alpha 2 Glycine Receptor 一抗
  • 描述
    兔多克隆抗体to alpha 2 Glycine Receptor
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Zebrafish
  • 免疫原

    Recombinant protein fragment corresponding to a region within N terminal amino acids 1 and 186 of alpha 2 Glycine Receptor.

  • 阳性对照
    • ICC/IF: formaldehyde fixed SKNSH cells. WB: 293T, A431, H1299, HeLa S3, HepG2, MOLT4 or Raji cell lysates. IHC-P: MDAMB468 xenograft.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab97628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/3000. Predicted molecular weight: 52 kDa.
IHC-P 1/100 - 1/500.

靶标

Anti-alpha 2 Glycine Receptor antibody 图像

  • Anti-alpha 2 Glycine Receptor antibody (ab97628) at 1/5000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 52 kDa
    10 % SDS PAGE
  • ab97628, at a 1/500 dilution, staining alpha 2 Glycine Receptor in paraffin embedded MDAMB468 xenograft by Immunohistochemistry.
  • ICC/IF image of ab97628 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab97628 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-alpha 2 Glycine Receptor antibody (ab97628)参考文献

This product has been referenced in:
  • Ren M  et al. A biofidelic 3D culture model to study the development of brain cellular systems. Sci Rep 6:24953 (2016). ICC/IF ; Rat . Read more (PubMed: 27112667) »

See 1 Publication for this product

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