Anti-Alpha 2 Antiplasmin抗体(ab62770)

概述

  • 产品名称Anti-Alpha 2 Antiplasmin抗体
    参阅全部 Alpha 2 Antiplasmin 一抗
  • 描述
    兔多克隆抗体to Alpha 2 Antiplasmin
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length human alpha 2 antiplasmin.

  • 阳性对照
    • This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2.

性能

应用

Our Abpromise guarantee covers the use of ab62770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 55 kDa.
ELISA 1/10000.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin.
  • 组织特异性Expressed by the liver and secreted in plasma.
  • 疾病相关Defects in SERPINF2 are the cause of alpha-2-plasmin inhibitor deficiency (APLID) [MIM:262850]. APLID is an autosomal recessive disorder resulting in severe hemorrhagic diathesis.
  • 序列相似性Belongs to the serpin family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A2AP antibody
    • A2AP_HUMAN antibody
    • AAP antibody
    • Alpha 2 antiplasmin antibody
    • Alpha 2 antiplasmin pigment epithelium derived factor antibody
    • Alpha 2 AP antibody
    • ALPHA 2 PI antibody
    • Alpha 2 plasmin inhibitor antibody
    • Alpha 2 plasmin inhibitor deficiency antibody
    • Alpha-2-antiplasmin antibody
    • Alpha-2-AP antibody
    • Alpha-2-PI antibody
    • Alpha-2-plasmin inhibitor antibody
    • Antiplasmin deficiency antibody
    • API antibody
    • Plasmin inhibitor deficiency antibody
    • PLI antibody
    • Serine (or cysteine) peptidase inhibitor, clade F, member 2 antibody
    • Serine (Or cysteine) peptidase inhibitor, clade F, member 2, isoform CRA_c antibody
    • Serine (or cysteine) proteinase inhibitor, clade F (alpha 2 antiplasmin pigment epithelium derived factor) member 2 antibody
    • Serine (Or cysteine) proteinase inhibitor, clade F, member 2 antibody
    • Serine or cysteine peptidase inhibitor clade F member 2 antibody
    • Serpin F2 antibody
    • Serpin peptidase inhibitor clade F antibody
    • Serpin peptidase inhibitor, clade F (alpha 2 antiplasmin pigment epithelium derived factor) member 2 antibody
    • SERPINF2 antibody
    see all

Anti-Alpha 2 Antiplasmin antibody 图像

  • ICC/IF image of ab62770 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab62770 at 5 µg/mL overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Alpha 2 Antiplasmin antibody (ab62770)参考文献

ab62770 has not yet been referenced specifically in any publications.

Product Wall

Our in house data shows only one band using this antibody. The western blot you are referring to is from one of our customers. Here they used brain lysates and observed 2 bands. The lower band is the band of interest at around 54 kDa. We do not know th...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (brain)
Loading amount 30 µg
Specification brain
Gel Running Conditions Reduced Denaturing (4-20% Tris-HCl gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Dr. Rachel Barker

Verified customer

提交于 Nov 12 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Cell (Brain)
Specification Brain
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Type Sandwich (Detection)
Username

Dr. Rachel Barker

Verified customer

提交于 Nov 01 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"