Anti-alpha 1 Sodium Potassium ATPase (phospho S23)抗体(ab74069)

概述

  • 产品名称Anti-alpha 1 Sodium Potassium ATPase (phospho S23)抗体
    参阅全部 alpha 1 Sodium Potassium ATPase 一抗
  • 描述
    兔多克隆抗体to alpha 1 Sodium Potassium ATPase (phospho S23)
  • 特异性ab74069 detects endogenous levels of alpha 1 Sodium Potassium ATPase only when phosphorylated at serine 23.
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from human alpha 1 Sodium Potassium ATPase around the phosphorylation site of serine 23 (K-K-SP-K-K).

  • 阳性对照
    • NIH/3T3 cells and rat brain extracts.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab74069 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab74069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 113 kDa (predicted molecular weight: 113 kDa).Can be blocked with alpha 1 Sodium Potassium ATPase peptide (ab180993).
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.

靶标

  • 功能This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
  • 序列相似性Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
  • 翻译后修饰Phosphorylation on Tyr-10 modulates pumping activity.
  • 细胞定位Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A1A1 antibody
    • AT1A1 antibody
    • AT1A1_HUMAN antibody
    • ATP1A1 antibody
    • Atpa-1 antibody
    • ATPase Na+/K+ transporting alpha 1 polypeptide antibody
    • ATPase Na+/K+ transporting subunit alpha 1 antibody
    • BC010319 antibody
    • EC 3.6.3.9 antibody
    • MGC3285 antibody
    • MGC38419 antibody
    • MGC51750 antibody
    • Na K ATPase alpha A catalytic polypeptide antibody
    • Na K ATPase catalytic subunit alpha A protein antibody
    • Na(+)/K(+) ATPase 1 antibody
    • Na(+)/K(+) ATPase alpha-1 subunit antibody
    • Na+, K+ ATPase alpha subunit antibody
    • Na+/K+ ATPase alpha 1 subunit antibody
    • Na+/K+ ATPase 1 antibody
    • Na,K ATPase alpha 1 subunit antibody
    • Nkaa1b antibody
    • Sodium potassium ATPase alpha 1 polypeptide antibody
    • Sodium pump 1 antibody
    • Sodium pump subunit alpha-1 antibody
    • sodium-potassium ATPase catalytic subunit alpha-1 antibody
    • Sodium/potassium-transporting ATPase subunit alpha-1 antibody
    see all

Anti-alpha 1 Sodium Potassium ATPase (phospho S23) antibody 图像

  • ab74069 at 1/500 dilution staining alpha 1 Sodium Potassium ATPase in NIH/3T3 cells by Immunofluorescence, in the absence or presence of the immunising phosphopeptide.
  • All lanes : Anti-alpha 1 Sodium Potassium ATPase (phospho S23) antibody (ab74069) at 1/500 dilution

    Lane 1 : Rat brain extracts
    Lane 2 : Rat brain extracts with immunising phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 113 kDa
    Observed band size : 113 kDa
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

Anti-alpha 1 Sodium Potassium ATPase (phospho S23) antibody (ab74069)参考文献

ab74069 has not yet been referenced specifically in any publications.

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