Alexa Fluor® 488荧光Anti-alpha 1 Sodium Potassium ATPase抗体[464.6] (ab197496)
Key features and details
- Alexa Fluor® 488 Mouse monoclonal [464.6] to alpha 1 Sodium Potassium ATPase
- Suitable for: ICC/IF
- Reacts with: Rat, Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Alexa Fluor® 488荧光Anti-alpha 1 Sodium Potassium ATPase抗体[464.6]
参阅全部 alpha 1 Sodium Potassium ATPase 一抗 -
描述
Alexa Fluor® 488荧光小鼠单克隆抗体[464.6] to alpha 1 Sodium Potassium ATPase -
宿主
Mouse -
偶联物
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Rat, Human
预测可用于: Mouse, Sheep, Rabbit, Dog, Pig, Xenopus laevis, Monkey -
免疫原
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- ICC/IF: HEK293 cells.
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常规说明
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
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纯度
IgG fraction -
克隆
单克隆 -
克隆编号
464.6 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab197496于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF | (1) |
1/100 - 1/250.
This product gave a positive signal in Hek293 cells fixed with 100% methanol (5 min) |
说明 |
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ICC/IF
1/100 - 1/250. This product gave a positive signal in Hek293 cells fixed with 100% methanol (5 min) |
靶标
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功能
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. -
序列相似性
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily. -
翻译后修饰
Phosphorylation on Tyr-10 modulates pumping activity. -
细胞定位
Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 403992 Dog
- Entrez Gene: 476 Human
- Entrez Gene: 11928 Mouse
- Entrez Gene: 397481 Pig
- Entrez Gene: 24211 Rat
- Entrez Gene: 443381 Sheep
- Entrez Gene: 399285 Xenopus laevis
- Omim: 182310 Human
see all -
别名
- A1A1 antibody
- AT1A1 antibody
- AT1A1_HUMAN antibody
see all
图片
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ab197496 staining alpha 1 Sodium Potassium ATPase in Hek293 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab197496 at 1/250 dilution (shown in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab197496 被引用在 3 文献中.
- Roy G et al. A novel bicistronic gene design couples stable cell line selection with a fucose switch in a designer CHO host to produce native and afucosylated glycoform antibodies. MAbs 10:416-430 (2018). PubMed: 29400603
- Yu SM et al. Substrate curvature affects the shape, orientation, and polarization of renal epithelial cells. Acta Biomater N/A:N/A (2018). PubMed: 30006316
- Honda K et al. Molecular architecture underlying fluid absorption by the developing inner ear. Elife 6:N/A (2017). IHC ; Mouse . PubMed: 28994389