Anti-Alpha 1 microglobulin抗体[BN11.10] (ab18513)

概述

  • 产品名称
    Anti-Alpha 1 microglobulin抗体[BN11.10]
    参阅全部 Alpha 1 microglobulin 一抗
  • 描述
    小鼠单克隆抗体[BN11.10] to Alpha 1 microglobulin
  • 经测试应用
    适用于: IHC-P, WB, ELISA, IP, RIA, Othermore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Full length native protein (purified) (Human and rat).

  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause.We would recommend antibody ab47980 as a replacement.

性能

应用

Our Abpromise guarantee covers the use of ab18513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 39 kDa.
ELISA Use a concentration of 0.2 - 2 µg/ml.
IP Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
AP Use at an assay dependent concentration.
Other Use at an assay dependent concentration.

靶标

  • 功能
    Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.
    Trypstatin is a trypsin inhibitor.
  • 组织特异性
    Expressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha-trypsin inhibitor is present in plasma and urine.
  • 序列相似性
    In the N-terminal section; belongs to the calycin superfamily. Lipocalin family.
    Contains 2 BPTI/Kunitz inhibitor domains.
  • 翻译后修饰
    The precursor is proteolytically processed into separately functioning proteins.
    3-hydroxykynurenine, an oxidized tryptophan metabolite that is common in biological fluids, reacts with Cys-53, Lys-111, Lys-137, and Lys-149 to form heterogeneous polycyclic chromophores including hydroxanthommatin. The reaction by alpha-1-microglobulin is autocatalytic; the human protein forms chromophore even when expressed in insect and bacterial cells. The chromophore can react with accessible cysteines forming non-reducible thioether cross-links with other molecules of alpha-1-microglobulin or with other proteins such as Ig alpha-1 chain C region 'Cys-352'.
    Heavy chains are interlinked with bikunin via a chondroitin 4-sulfate bridge to the their C-terminal aspartate.
    Addition of glycosaminoglycan chondroitin sulfate, allows cross-linking between the different components.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A1M antibody
    • Alpha 1 microglobulin/bikunin precursor antibody
    • Alpha 1 microglycoprotein antibody
    • Alpha-1 microglycoprotein antibody
    • Alpha-1-microglobulin antibody
    • AMBP antibody
    • AMBP_HUMAN antibody
    • Bikunin antibody
    • Complex-forming glycoprotein heterogeneous in charge antibody
    • EDC1 antibody
    • Growth inhibiting protein 19 antibody
    • HCP antibody
    • HI-30 antibody
    • HI30 antibody
    • IATIL antibody
    • ITI antibody
    • ITI-LC antibody
    • ITIL antibody
    • Microglobulin (alpha 1) antibody
    • Protein AMBP antibody
    • Protein HC antibody
    • Trypstatin antibody
    • Uristatin antibody
    • Uronic-acid-rich protein antibody
    • UTI antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal liver tissues (left) and ICC tissues (right) labelling Alpha 1 microglobulin with ab18513 at 1/200. Alpha 1 microglobulin positive cells were mainly detected in the cytosol of hepatocytes in normal liver tissues and peri-tumoral tissues, but not in ICC tissues. T, ICC tissue; N, peri-tumoral tissue. Magnifications: 200X.

文献

This product has been referenced in:
  • Wang AG  et al. Identification of intrahepatic cholangiocarcinoma related genes by comparison with normal liver tissues using expressed sequence tags. Biochem Biophys Res Commun 345:1022-32 (2006). IHC-P ; Human . Read more (PubMed: 16712791) »

See 1 Publication for this product

客户评价及客户问答


The immunogen used to produce Anti-Alpha 1 microglobulin antibody [BN11.10] cat# ab18513 is human and rat alpha-1-microglobulin, native, full length, purified from urine.
I confirm it corresponds to the alpha-1-microglobulin fragment: UniProt...

Read More

Thank you for contacting us. Indeed, the Alpha 1 microglobulin antibody ab18513 has been tested in an indirect ELISA protocol. Please find attached our detailed protocol. Protocols for other ELISA techniques as well as other applications can also...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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