Anti-Alpha 1 microglobulin抗体(ab47980)


  • 产品名称Anti-Alpha 1 microglobulin抗体
    参阅全部 Alpha 1 microglobulin 一抗
  • 描述
    兔多克隆抗体to Alpha 1 microglobulin
  • 经测试应用适用于: WB, ICC/IF, IHC-P, ELISA, RIAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Human Alpha 1 Microglobulin



Our Abpromise guarantee covers the use of ab47980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 39 kDa.
ICC/IF Use a concentration of 1 - 5 µg/ml.
IHC-P Use a concentration of 4 µg/ml.
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.


  • 功能Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.
    Trypstatin is a trypsin inhibitor.
  • 组织特异性Expressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha-trypsin inhibitor is present in plasma and urine.
  • 序列相似性In the N-terminal section; belongs to the calycin superfamily. Lipocalin family.
    Contains 2 BPTI/Kunitz inhibitor domains.
  • 翻译后修饰The precursor is proteolytically processed into separately functioning proteins.
    3-hydroxykynurenine, an oxidized tryptophan metabolite that is common in biological fluids, reacts with Cys-53, Lys-111, Lys-137, and Lys-149 to form heterogeneous polycyclic chromophores including hydroxanthommatin. The reaction by alpha-1-microglobulin is autocatalytic; the human protein forms chromophore even when expressed in insect and bacterial cells. The chromophore can react with accessible cysteines forming non-reducible thioether cross-links with other molecules of alpha-1-microglobulin or with other proteins such as Ig alpha-1 chain C region 'Cys-352'.
    Heavy chains are interlinked with bikunin via a chondroitin 4-sulfate bridge to the their C-terminal aspartate.
    Addition of glycosaminoglycan chondroitin sulfate, allows cross-linking between the different components.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A1M antibody
    • Alpha 1 microglobulin/bikunin precursor antibody
    • Alpha 1 microglycoprotein antibody
    • Alpha-1 microglycoprotein antibody
    • Alpha-1-microglobulin antibody
    • AMBP antibody
    • AMBP_HUMAN antibody
    • Bikunin antibody
    • Complex-forming glycoprotein heterogeneous in charge antibody
    • EDC1 antibody
    • Growth inhibiting protein 19 antibody
    • HCP antibody
    • HI-30 antibody
    • HI30 antibody
    • IATIL antibody
    • ITI antibody
    • ITI-LC antibody
    • ITIL antibody
    • Microglobulin (alpha 1) antibody
    • Protein AMBP antibody
    • Protein HC antibody
    • Trypstatin antibody
    • Uristatin antibody
    • Uronic-acid-rich protein antibody
    • UTI antibody
    see all

Anti-Alpha 1 microglobulin antibody 图像

  • ICC/IF image of ab47980 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47980, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab47980 Microglobulin alpha 1 in human liver.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-Alpha 1 microglobulin antibody (ab47980)参考文献

ab47980 has not yet been referenced specifically in any publications.

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