特异性Alpha-Fetoprotein (AFP) is a single chain glycoprotein of 70 kDa that is normally expressed in the fetal liver, gastrointestinal tract and yolk sac.
This antibody recognizes an epitope of alpha - Fetoprotein present in human and several other species.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections.
No further titration is required.
We suggest an incubation period of 30-60 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user.
功能Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
组织特异性Plasma. Synthesized by the fetal liver and yolk sac.
序列相似性Belongs to the ALB/AFP/VDB family. Contains 3 albumin domains.
发展阶段Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
翻译后修饰Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence. Sulfated.