Anti-alpha 1 Fetoprotein抗体[AFP-11] (ab3969)

概述

  • 产品名称Anti-alpha 1 Fetoprotein抗体[AFP-11]
    参阅全部 alpha 1 Fetoprotein 一抗
  • 描述
    小鼠单克隆抗体[AFP-11] to alpha 1 Fetoprotein
  • 特异性The antibody reacts with human alpha-Fetoprotein (AFP), an oncofetal antigen of 70 kDa. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.
  • 经测试应用适用于: ICC/IF, IHC-P, RIA, Sandwich ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length native protein (purified) corresponding to Human alpha 1 Fetoprotein.

性能

应用

Our Abpromise guarantee covers the use of ab3969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/200. PubMed: 19497803
IHC-P Use a concentration of 10 µg/ml.
RIA Use at an assay dependent concentration.
Sandwich ELISA Use at an assay dependent concentration.

靶标

  • 功能Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
  • 组织特异性Plasma. Synthesized by the fetal liver and yolk sac.
  • 序列相似性Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • 发展阶段Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
  • 翻译后修饰Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
    Sulfated.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Afp antibody
    • Alpha fetoglobulin antibody
    • Alpha fetoprotein antibody
    • Alpha fetoprotein precursor antibody
    • Alpha-1-fetoprotein antibody
    • Alpha-fetoglobulin antibody
    • Alpha-fetoprotein antibody
    • alpha-fetoprotein, Hereditary persistence of, included antibody
    • FETA antibody
    • FETA_HUMAN antibody
    • Hereditary persistence of alpha fetoprotein antibody
    • HPAFP antibody
    see all

Anti-alpha 1 Fetoprotein antibody [AFP-11] 图像

  • Immunohistochemical analysis of paraffin-embedded hepatocellular carcinoma (fetal liver) sections labelling alpha 1 Fetoprotein with ab3969 at 10 µg/mL. 

Anti-alpha 1 Fetoprotein antibody [AFP-11] (ab3969)参考文献

This product has been referenced in:
  • Zhang A  et al. A Chemiluminescent Protein Microarray Method for Determining the Seroglycoid Fucosylation Index. Sci Rep 6:31132 (2016). Human . Read more (PubMed: 27528397) »
  • Moore RN & Moghe PV Expedited growth factor-mediated specification of human embryonic stem cells toward the hepatic lineage. Stem Cell Res 3:51-62 (2009). ICC/IF ; Human . Read more (PubMed: 19497803) »

See all 3 Publications for this product

Product Wall

Having reviewed our catalog of anti-alpha 1 Fetoprotein antibodies suitable for use in ELISA, I have found the following 2 pairs which have been verified by us. AB8276 pairs with ab37207. More information about these items may be found at: Ab8276...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"