概述
- 产品名称Anti-alpha 1 Fetoprotein抗体
参阅全部 alpha 1 Fetoprotein 一抗 - 描述兔多克隆抗体to alpha 1 Fetoprotein
- 经测试应用适用于: WBmore details
- 种属反应性与反应: Human
预测可用于: Mouse, Rat, Horse, Cow, Pig
- 免疫原
Synthetic peptide within Mouse alpha 1 Fetoprotein aa 500-600 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
(Peptide available asab135000) - 阳性对照
- This antibody gave a positive signal in Human Fetal Liver tissue lysate.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- 存储溶液pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... - 纯度Immunogen affinity purified
- 克隆多克隆
- 同种型IgG
- 研究领域
相关产品
- Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab115133 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 67 kDa). |
靶标
- 功能Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
- 组织特异性Plasma. Synthesized by the fetal liver and yolk sac.
- 序列相似性Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. - 发展阶段Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
- 翻译后修饰Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
Sulfated. - 细胞定位Secreted.
- Information by UniProt
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数据库链接
- Entrez Gene: 506011 Cow
- Entrez Gene: 00034185 Horse
- Entrez Gene: 174 Human
- Entrez Gene: 11576 Mouse
- Entrez Gene: 24177 Rat
- Omim: 104150 Human
- SwissProt: Q3SZ57 Cow
- SwissProt: P49066 Horse
see all -
别名
- Afp antibody
- AFPD antibody
- Alpha fetoglobulin antibody
see all
Anti-alpha 1 Fetoprotein antibody 图像
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Western blot - Anti-alpha 1 Fetoprotein antibody (ab115133)Anti-alpha 1 Fetoprotein antibody (ab115133) at 1 µg/ml + Liver (Human) Tissue Lysate - fetal normal tissue (ab29890) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 67 kDa
Observed band size : 69 kDa (why is the actual band size different from the predicted?)
Additional bands at : 40 kDa,48 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 10 seconds
Anti-alpha 1 Fetoprotein antibody (ab115133)参考文献
ab115133 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"