Anti-alpha 1 Fetoprotein抗体(ab115133)

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概述

  • 产品名称
    Anti-alpha 1 Fetoprotein抗体
    参阅全部 alpha 1 Fetoprotein 一抗
  • 描述
    兔多克隆抗体to alpha 1 Fetoprotein
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Cow, Pig
  • 免疫原

    Synthetic peptide within Mouse alpha 1 Fetoprotein aa 500-600 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    (Peptide available as ab135000)

  • 阳性对照
    • This antibody gave a positive signal in Human Fetal Liver tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab115133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 67 kDa).

靶标

  • 功能
    Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
  • 组织特异性
    Plasma. Synthesized by the fetal liver and yolk sac.
  • 序列相似性
    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • 发展阶段
    Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
  • 翻译后修饰
    Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
    Sulfated.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • Afp antibody
    • AFPD antibody
    • Alpha fetoglobulin antibody
    • Alpha fetoprotein antibody
    • Alpha fetoprotein precursor antibody
    • Alpha-1-fetoprotein antibody
    • Alpha-fetoglobulin antibody
    • Alpha-fetoprotein antibody
    • alpha-fetoprotein, Hereditary persistence of, included antibody
    • FETA antibody
    • FETA_HUMAN antibody
    • Hereditary persistence of alpha fetoprotein antibody
    • HPAFP antibody
    see all

图片

  • Western blot - Anti-alpha 1 Fetoprotein antibody (ab115133)
    Western blot - Anti-alpha 1 Fetoprotein antibody (ab115133)
    Anti-alpha 1 Fetoprotein antibody (ab115133) at 1 µg/ml + Liver (Human) Tissue Lysate - fetal normal tissue (ab29890) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 67 kDa
    Observed band size: 69 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 40 kDa, 48 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 10 seconds

实验方案

文献

ab115133 has not yet been referenced specifically in any publications.

发表研究结果有使用 ab115133？请让我们知道，以便我们可以引用本数据表中的参考文章。

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