Anti-alpha 1 Fetoprotein抗体[4A3] (ab10071)

概述

  • 产品名称Anti-alpha 1 Fetoprotein抗体[4A3]
    参阅全部 alpha 1 Fetoprotein 一抗
  • 描述
    小鼠单克隆抗体[4A3] to alpha 1 Fetoprotein
  • 特异性ab10071 and 10072 react with different determinants of human a-fetoprotein. They do not react with albumin.
  • 经测试应用适用于: Sandwich ELISA, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein (purified) (Human).

  • 常规说明Concentration varies from lot to lot and can be provided on request.

性能

应用

Our Abpromise guarantee covers the use of ab10071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [5H7] to alpha 1 Fetoprotein (HRP) (ab10072). Can be used as capture antibody.
ELISA Use at an assay dependent dilution.

靶标

  • 功能Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
  • 组织特异性Plasma. Synthesized by the fetal liver and yolk sac.
  • 序列相似性Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • 发展阶段Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
  • 翻译后修饰Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
    Sulfated.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Afp antibody
    • Alpha fetoglobulin antibody
    • Alpha fetoprotein antibody
    • Alpha fetoprotein precursor antibody
    • Alpha-1-fetoprotein antibody
    • Alpha-fetoglobulin antibody
    • Alpha-fetoprotein antibody
    • alpha-fetoprotein, Hereditary persistence of, included antibody
    • FETA antibody
    • FETA_HUMAN antibody
    • Hereditary persistence of alpha fetoprotein antibody
    • HPAFP antibody
    see all

Anti-alpha 1 Fetoprotein antibody [4A3] (ab10071)参考文献

This product has been referenced in:
  • Schmelzer E  et al. Three-dimensional perfusion bioreactor culture supports differentiation of human fetal liver cells. Tissue Eng Part A 16:2007-16 (2010). Sandwich ELISA ; Human . Read more (PubMed: 20088704) »

See 1 Publication for this product

Product Wall

Thanks for your reply, and I am sorry that I don't have a better explanation for this problem. If there is anything else that we can do for you, please let me know and I'll be happy to help.

I just wanted to thank you for completing our follow-up survey recently, and I'm sorry to see that this problem persisted even with the replacement vial of antibody. Since the results are still not satisfactory, I would be happy to issue a credit or re...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Cell (Liver cell culture)
Specification Liver cell culture
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Type Sandwich (Capture)
Username

Dr. Eva Schmelzer

Verified customer

提交于 Jul 20 2010

Thank you for your enquiry. Unless a dilution is listed on the data sheet, we do not know the dilution to use for sandwich ELISA. A few things to keep in mind are that typically you do not need a lot of antibody to do an ELISA, just enough to coat the ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"