重组Anti-CTNNA1抗体[EP1793Y] (ab51032)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1793Y] to CTNNA1
- Suitable for: WB, IHC-P, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
概述
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产品名称
Anti-CTNNA1抗体[EP1793Y]
参阅全部 CTNNA1 一抗 -
描述
兔单克隆抗体[EP1793Y] to CTNNA1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, IPmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Mouse heart and kidney lysate. Rat brain and kidney lysate. HeLa, A431 and HUVEC whole cell lysate. IHC-P: Rat and human stomach tissue and mouse liver tissue IP: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1793Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab51032于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (4) |
1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
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IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IP |
1/100.
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说明 |
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WB
1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa). |
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
1/100. |
靶标
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功能
Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation. -
组织特异性
Expressed ubiquitously in normal tissues. -
序列相似性
Belongs to the vinculin/alpha-catenin family. -
翻译后修饰
Sumoylated. -
细胞定位
Cell membrane and Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell membrane. Cell junction. Found at cell-cell boundaries and probably at cell-matrix boundaries. - Information by UniProt
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数据库链接
- Entrez Gene: 1495 Human
- Entrez Gene: 12385 Mouse
- Entrez Gene: 307505 Rat
- Omim: 116805 Human
- SwissProt: P35221 Human
- SwissProt: P26231 Mouse
- Unigene: 445981 Human
- Unigene: 656653 Human
see all -
别名
- Alpha E-catenin antibody
- Cadherin associated protein 102kDa antibody
- Cadherin associated protein antibody
see all
图片
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ab190685 at 1/100 dilution immunoprecipitating CTNNA1 in Jurkat HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 1 (input): HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg
Lane 2 (+): HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab51032 in HeLa whole cell lysate
For western blotting, ab51032 at 1/500 dilution and VeriBlot for IP Detection Reagent (HRP)(ab131366) at 1/1000 dilution were used.Blocking and diluting buffer: 5% NFDM /TBST.
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Paraffin-embedded rat stomach tissue stained for CTNNA1 with ab51032 at a 1/100 dilution in immunohistochemical analysis. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as a secondary antibody and Hematoxylin used as a counterstain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was performed for 20 minutes.
Positive staining was seen on rat stomach.
The section was incubated with ab51032 for 30 minutes at room temperature.
The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
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Paraffin-embedded mouse liver tissue stained for CTNNA1 with ab51032 at a 1/100 dilution in immunohistochemical analysis. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as a secondary antibody and Hematoxylin used as a counterstain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was performed for 20 minutes.
Positive staining was seen on mouse liver.
The section was incubated with ab51032 for 30 minutes at room temperature.
The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
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Paraffin-embedded human stomach tissue stained for CTNNA1 with ab51032 at a 1/100 dilution in immunohistochemical analysis. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as a secondary antibody and Hematoxylin used as a counterstain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was performed for 20 minutes.
Positive staining was seen on human stomach.
The section was incubated with ab51032 for 30 minutes at room temperature.
The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
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All lanes : Anti-CTNNA1 antibody [EP1793Y] (ab51032) at 1/10000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Rat brain lysate
Lane 4 : Rat kidney lysate
Lane 5 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 6 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
Lane 7 : HUVEC (Human umbilical vein endothelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 100 kDa
Observed band size: 100 kDaExpsure time
Lane 1-4: 180 seconds
Lane 5,7: 40 seconds
Lane 6: 5 seconds
Blocking/diluting buffer and concentration: 5% NFDM/TBST
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Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: CTNNA1 HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HEK293 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab51032 observed at 100 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab51032 was shown to recognize CTNNA1 in wild-type cells as signal was lost at the expected MW in CTNNA1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CTNNA1 knockout samples were subjected to SDS-PAGE. Ab51032 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/50000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (31)
ab51032 被引用在 31 文献中.
- Hu X et al. Effect of high-throughput screening of circRNA01724 on a rat model of myocardial ischemia ventricular arrhythmia. J Thorac Dis 14:1172-1182 (2022). PubMed: 35572876
- Kilinc AN et al. Integrin-linked kinase tunes cell-cell and cell-matrix adhesions to regulate the switch between apoptosis and EMT downstream of TGFß1. Mol Biol Cell 32:402-412 (2021). PubMed: 33405954
- Song J et al. Apatinib suppresses the migration, invasion and angiogenesis of hepatocellular carcinoma cells by blocking VEGF and PI3K/AKT signaling pathways. Mol Med Rep 23:N/A (2021). PubMed: 33846786
- Pavel M et al. a-Catenin levels determine direction of YAP/TAZ response to autophagy perturbation. Nat Commun 12:1703 (2021). PubMed: 33731717
- Zhang N et al. Electrophysiologic and Morphologic Strain Differences in a Low-Dose NaIO3-Induced Retinal Pigment Epithelium Damage Model. Transl Vis Sci Technol 10:10 (2021). PubMed: 34251426