Anti-alpha 1 Catenin抗体(ab6299)

Abreviews (1)Q&A (2)Specific References (1)
Customer reviews and FAQs

概述

  • 产品名称
    Anti-alpha 1 Catenin抗体
    参阅全部 alpha 1 Catenin 一抗
  • 描述
    兔多克隆抗体to alpha 1 Catenin
  • 特异性
    Reacts in dot blot immunoassay with alpha-catenin peptide amino acids 890-901 conjugated to BSA. Reacts with a 102 kD protein in extracts of Madin-Darby Bovine Kidney (MDBK) cultured cell line using immunoblotting. The antiserum shows no cross-reactivity with beta-catenin peptide (amino acids 768-781)conjugated to BSA. Specific staining in immunoblotting is inhibited by pre-incubating the diluted anti-serum with the alpha-catenin peptide.
  • 经测试应用
    适用于: WB, IHC-Fr, Dot blot, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Cow, Human
  • 免疫原

    Synthetic peptide:

    HVNPVQALSEFK

    conjugated to KLH, corresponding to amino acids 890-901 of Human and mouse α Catenin.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照
    • ICC or WB: MDBK epithelial cells IHC-Fr: bovine kidney

性能

应用

Our Abpromise guarantee covers the use of ab6299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-Fr
Dot blot
ICC/IF
  • 应用说明
    Dot: Use at an assay dependent dilution.
    ICC/IF: 1/2000 (indirect. See recommended protocol).
    IHC-Fr: 1/2000.
    WB: 1/4000.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • 靶标

    • 功能
      Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.
    • 组织特异性
      Expressed ubiquitously in normal tissues.
    • 序列相似性
      Belongs to the vinculin/alpha-catenin family.
    • 翻译后修饰
      Sumoylated.
    • 细胞定位
      Cell membrane and Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell membrane. Cell junction. Found at cell-cell boundaries and probably at cell-matrix boundaries.
    • Information by UniProt
    • 数据库链接
      • 别名
        • Alpha E-catenin antibody
        • Cadherin associated protein 102kDa antibody
        • Cadherin associated protein antibody
        • Cadherin-associated protein antibody
        • CAP 102 antibody
        • CAP102 antibody
        • Catenin (cadherin associated protein) alpha 1 102kDa antibody
        • Catenin (cadherin associated protein), alpha 1, 102kDa antibody
        • Catenin alpha 1 antibody
        • Catenin alpha-1 antibody
        • CTNA1_HUMAN antibody
        • CTNNA 1 antibody
        • Ctnna1 antibody
        • FLJ36832 antibody
        • FLJ52416 antibody
        • MDPT2 antibody
        • NY REN 13 antigen antibody
        • OTTHUMP00000224141 antibody
        • OTTHUMP00000224147 antibody
        • Renal carcinoma antigen NY REN 13 antibody
        • Renal carcinoma antigen NY-REN-13 antibody
        see all

      Anti-alpha 1 Catenin antibody (ab6299)参考文献

      This product has been referenced in:
      • Poehland R  et al. Qualitative and quantitative differences of cytoskeleton proteins in embryos produced in vitro, in vivo, and by somatic nuclear transfer. Mol Reprod Dev 75:1109-19 (2008). WB, ICC/IF ; Cow . Read more (PubMed: 18095314) »

      See 1 Publication for this product

      发表研究结果有使用 ab6299？请让我们知道，以便我们可以引用本数据表中的参考文章。

      Product Wall

      提交 ab6299 的 abreview 提交 ab6299 的问题

      3 Abreviews or Q&A

      10
      Votes: Highest First

      Western blot abreview for Anti-alpha 1 Catenin antibody

       Good
      Abreviews
      Abcam guarantees this product to work in the species/application used in this Abreview.
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (DLD1)
      Loading amount
      20 µg
      Specification
      DLD1
      Treatment
      boiled with laemmli sample buffer
      Gel Running Conditions
      Reduced Denaturing (10%)
      Blocking step
      Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
      Read More
      abreview image
      Username

      Ms. Vigil Yu

      Verified customer

      提交于 Aug 23 2012

      is this antibody also working in MDCK cells?does it work for immunoprecipitation studies?

      This antibody has not been tested in IP to our knowledge. We don't have any information as to whether the antibody will work in MDCK cells. All the information we have available on this antibody is on the datasheet.

      Is it also reactive with chicken cells ? Can it be used on cells in culture wells (cytochemistry) ?

      This antibody has been tested for cross-reactivity against mammalian species only, hence its reactivity with chicken is unknown. It has not been specifically tested in the application you describe, however it would be expected to work with mammalian ce...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

      注册