Anti-alpha 1 Catenin抗体(ab6299)

概述

  • 产品名称Anti-alpha 1 Catenin抗体
    参阅全部 alpha 1 Catenin 一抗
  • 描述
    兔多克隆抗体to alpha 1 Catenin
  • 特异性Reacts in dot blot immunoassay with alpha-catenin peptide amino acids 890-901 conjugated to BSA. Reacts with a 102 kD protein in extracts of Madin-Darby Bovine Kidney (MDBK) cultured cell line using immunoblotting. The antiserum shows no cross-reactivity with beta-catenin peptide (amino acids 768-781)conjugated to BSA. Specific staining in immunoblotting is inhibited by pre-incubating the diluted anti-serum with the alpha-catenin peptide.
  • 经测试应用适用于: WB, IHC-Fr, Dot blot, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Cow, Human
  • 免疫原

    Synthetic peptide:

    HVNPVQALSEFK

    conjugated to KLH, corresponding to amino acids 890-901 of Human and mouse α Catenin.

  • 阳性对照
    • ICC or WB: MDBK epithelial cells IHC-Fr: bovine kidney

性能

应用

Our Abpromise guarantee covers the use of ab6299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-Fr
Dot blot
ICC/IF
  • 应用说明Dot: Use at an assay dependent dilution.
    ICC/IF: 1/2000 (indirect. See recommended protocol).
    IHC-Fr: 1/2000.
    WB: 1/4000.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.
    • 组织特异性Expressed ubiquitously in normal tissues.
    • 序列相似性Belongs to the vinculin/alpha-catenin family.
    • 翻译后修饰Sumoylated.
    • 细胞定位Cell membrane and Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell membrane. Cell junction. Found at cell-cell boundaries and probably at cell-matrix boundaries.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Alpha E-catenin antibody
      • Cadherin associated protein 102kDa antibody
      • Cadherin associated protein antibody
      • Cadherin-associated protein antibody
      • CAP 102 antibody
      • CAP102 antibody
      • Catenin (cadherin associated protein) alpha 1 102kDa antibody
      • Catenin (cadherin associated protein), alpha 1, 102kDa antibody
      • Catenin alpha 1 antibody
      • Catenin alpha-1 antibody
      • CTNA1_HUMAN antibody
      • CTNNA 1 antibody
      • Ctnna1 antibody
      • FLJ36832 antibody
      • FLJ52416 antibody
      • NY REN 13 antigen antibody
      • OTTHUMP00000224141 antibody
      • OTTHUMP00000224147 antibody
      • Renal carcinoma antigen NY REN 13 antibody
      • Renal carcinoma antigen NY-REN-13 antibody
      see all

    Anti-alpha 1 Catenin antibody (ab6299)参考文献

    This product has been referenced in:
    • Poehland R  et al. Qualitative and quantitative differences of cytoskeleton proteins in embryos produced in vitro, in vivo, and by somatic nuclear transfer. Mol Reprod Dev 75:1109-19 (2008). WB, ICC/IF ; Cow . Read more (PubMed: 18095314) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (DLD1)
    Loading amount 20 µg
    Specification DLD1
    Treatment boiled with laemmli sample buffer
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Ms. Vigil Yu

    Verified customer

    提交于 Aug 23 2012

    This antibody has not been tested in IP to our knowledge. We don't have any information as to whether the antibody will work in MDCK cells. All the information we have available on this antibody is on the datasheet.

    This antibody has been tested for cross-reactivity against mammalian species only, hence its reactivity with chicken is unknown. It has not been specifically tested in the application you describe, however it would be expected to work with mammalian ce...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"