Anti-alpha 1 Antitrypsin抗体[G11] (ab9400)
Key features and details
- Mouse monoclonal [G11] to alpha 1 Antitrypsin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-alpha 1 Antitrypsin抗体[G11]
参阅全部 alpha 1 Antitrypsin 一抗 -
描述
小鼠单克隆抗体[G11] to alpha 1 Antitrypsin -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Full length protein corresponding to Human alpha 1 Antitrypsin.
Database link: P01009 -
常规说明
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR3176514-1 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
G11 -
骨髓瘤
NS0/1 -
同种型
IgG1 -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/1000 - 1/5000.
|
说明 |
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WB
1/1000 - 1/5000. |
靶标
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功能
Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE). -
组织特异性
Ubiquitous. Expressed in leukocytes and plasma. -
疾病相关
Alpha-1-antitrypsin deficiency -
序列相似性
Belongs to the serpin family. -
结构域
The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable. -
翻译后修饰
N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418. -
细胞定位
Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 5265 Human
- Omim: 107400 Human
- SwissProt: P01009 Human
- Unigene: 525557 Human
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别名
- A1A antibody
- A1AT antibody
- A1AT_HUMAN antibody
see all
图片
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Anti-alpha 1 Antitrypsin antibody [G11] (ab9400) at 1/1000 dilution + Albumin depleted human patient sera, tissue lysate at 50 µg/ml
Secondary
HRP conjugated goat anti-mouse IgG (H&L). at 1/5000 dilution
Observed band size: 52 kDa why is the actual band size different from the predicted?
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Western blot using ab9400 at a concentration of 0.5 µg/ml.
Lane 1 - purified alpha 1 antitrypsin
lane 2 - human plasma, 2 µl
lane S - standard proteins
A - SDS-PAGE with Coomasie staining
B - immunoblot with ab9400.Western blot using ab9400 at a concentration of 0.5 µg/ml.
Lane 1 - purified alpha 1 antitrypsin
lane 2 - human plasma, 2 µl
lane S - standard proteins
A - SDS-PAGE with Coomasie staining
B - immunoblot with ab9400.
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab9400 被引用在 14 文献中.
- Patil K et al. Quantitative mass spectrometric analysis to unravel glycoproteomic signature of follicular fluid in women with polycystic ovary syndrome. PLoS One 14:e0214742 (2019). PubMed: 30946770
- Cagnone M et al. A Pilot Study to Investigate the Balance between Proteases and a1-Antitrypsin in Bronchoalveolar Lavage Fluid of Lung Transplant Recipients. High Throughput 8:N/A (2019). PubMed: 30781848
- Esteghamat F et al. CELA2A mutations predispose to early-onset atherosclerosis and metabolic syndrome and affect plasma insulin and platelet activation. Nat Genet 51:1233-1243 (2019). PubMed: 31358993
- Henneuse A et al. a1 -antitrypsin Pittsburgh and plasmin-mediated proteolysis. J Thromb Haemost 14:2023-2026 (2016). PubMed: 27468101
- Kang H et al. Fluid shear stress promotes proprotein convertase-dependent activation of MT1-MMP. Biochem Biophys Res Commun 460:596-602 (2015). PubMed: 25800869