重组Anti-alpha 1 Antitrypsin抗体[EPR17087-50] (ab207303)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17087-50] to alpha 1 Antitrypsin
- Suitable for: IHC-P, WB, IP, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-alpha 1 Antitrypsin抗体[EPR17087-50]
参阅全部 alpha 1 Antitrypsin 一抗 -
描述
兔单克隆抗体[EPR17087-50] to alpha 1 Antitrypsin -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, IP, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Full length native protein (purified) corresponding to Human alpha 1 Antitrypsin.
Database link: P01009 -
阳性对照
- WB: Human liver, heart, kidney, spleen and fetal liver lysates. IHC-P: Human hepatocellular carcinoma and liver tissues. ICC/IF: HepG2 and K562 cells. IP: HepG2 whole cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR17087-50 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab207303于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (2) |
1/400. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
WB |
1/5000. Detects a band of approximately 51, 55 kDa (predicted molecular weight: 46 kDa).
|
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IP |
1/20.
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ICC/IF |
1/50.
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说明 |
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IHC-P
1/400. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/5000. Detects a band of approximately 51, 55 kDa (predicted molecular weight: 46 kDa). |
IP
1/20. |
ICC/IF
1/50. |
靶标
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功能
Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE). -
组织特异性
Ubiquitous. Expressed in leukocytes and plasma. -
疾病相关
Alpha-1-antitrypsin deficiency -
序列相似性
Belongs to the serpin family. -
结构域
The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable. -
翻译后修饰
N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418. -
细胞定位
Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 5265 Human
- Omim: 107400 Human
- SwissProt: P01009 Human
- Unigene: 525557 Human
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别名
- A1A antibody
- A1AT antibody
- A1AT_HUMAN antibody
see all
图片
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All lanes : Anti-alpha 1 Antitrypsin antibody [EPR17087-50] (ab207303) at 1/5000 dilution
Lane 1 : Human liver lysate
Lane 2 : Human heart lysate
Lane 3 : Human kidney lysate
Lane 4 : Human spleen lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 46 kDa
Observed band size: 51,55 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
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Anti-alpha 1 Antitrypsin antibody [EPR17087-50] (ab207303) at 1/5000 dilution + Human fetal liver lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG. at 1/10000 dilution
Predicted band size: 46 kDa
Observed band size: 51,55 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling alpha 1 Antitrypsin with ab207303 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on cancer cells of human hepatocellular carcinoma is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human liver tissue labeling alpha 1 Antitrypsin with ab207303 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human liver is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling alpha 1 Antitrypsin with ab207303 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HepG2 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse mAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse Alexa Fluor® 594 (ab150120) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab207303 at 1/50 dilution, followed by Goat Anti-Mouse Alexa Fluor® 594 (ab150120) secondary antibody at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin mouse mAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) seconday antibody at 1/1000 dilution. -
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling alpha 1 Antitrypsin with ab207303 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on K562 cell line. The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin mouse mAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab207303 at 1/50 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin mouse mAb (ab7291) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution. -
alpha 1 Antitrypsin was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma) whole cell lysate with ab207303 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab207303 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HepG2 whole cell lysate 10ug (Input).
Lane 2: ab207303 IP in HepG2 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207303 in HepG2 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (9)
ab207303 被引用在 9 文献中.
- Li JY et al. New and effective method to develop primary hepatocytes from liver cancer patients. Exp Biol Med (Maywood) 247:972-981 (2022). PubMed: 35470702
- Fu W et al. Cellular features of localized microenvironments in human meniscal degeneration: a single-cell transcriptomic study. Elife 11:N/A (2022). PubMed: 36548025
- Xu D et al. Cullin 2-RBX1 E3 ligase and USP2 regulate antithrombin ubiquitination and stability. FASEB J 35:e21800 (2021). PubMed: 34324733
- Wei J et al. HRD1-mediated METTL14 degradation regulates m6A mRNA modification to suppress ER proteotoxic liver disease. Mol Cell 81:5052-5065.e6 (2021). PubMed: 34847358
- Baldan-Martin M et al. Comprehensive Proteomic Profiling of Pressure Ulcers in Patients with Spinal Cord Injury Identifies a Specific Protein Pattern of Pathology. Adv Wound Care (New Rochelle) 9:277-294 (2020). PubMed: 32226651
- Bai J et al. iTRAQ-based proteomic analysis reveals potential regulatory networks in dust mite-related asthma treated with subcutaneous allergen immunotherapy. Mol Med Rep 22:3607-3620 (2020). PubMed: 32901873
- Jiang L & Hu LG Serpin peptidase inhibitor clade A member 1-overexpression in gastric cancer promotes tumor progression in vitro and is associated with poor prognosis. Oncol Lett 20:278 (2020). PubMed: 33014156
- Zhao Z et al. Silence of a1-Antitrypsin Inhibits Migration and Proliferation of Triple Negative Breast Cancer Cells. Med Sci Monit 24:6851-6860 (2018). PubMed: 30260937
- Sarabhai T et al. Serum a-1 Antitrypsin (AAT) antagonizes intrinsic apoptosis induction in neutrophils from patients with systemic inflammatory response syndrome. PLoS One 12:e0177450 (2017). PubMed: 28493974