Anti-alpha 1 Antitrypsin抗体(ab9373)

概述

  • 产品名称Anti-alpha 1 Antitrypsin抗体
    参阅全部 alpha 1 Antitrypsin 一抗
  • 描述
    兔多克隆抗体to alpha 1 Antitrypsin
  • 特异性The antibody is specific against human alpha-1-antitrypsin.
  • 经测试应用适用于: ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mammal
  • 免疫原

    Alpha-1-antitrypsin isolated from human serum.

  • 阳性对照
    • IHC-P: Human tonsil tissue. ICC/IF: HepG2 cells.

性能

应用

Our Abpromise guarantee covers the use of ab9373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
    Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
  • 组织特异性Ubiquitous. Expressed in leukocytes and plasma.
  • 疾病相关Alpha-1-antitrypsin deficiency
  • 序列相似性Belongs to the serpin family.
  • 结构域The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
  • 翻译后修饰N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
    Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418.
  • 细胞定位Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A1A antibody
    • A1AT antibody
    • A1AT_HUMAN antibody
    • AAT antibody
    • Alpha 1 antiproteinase antibody
    • Alpha 1 antitrypsin antibody
    • Alpha 1 antitrypsin null antibody
    • Alpha 1 protease inhibitor antibody
    • Alpha-1 protease inhibitor antibody
    • Alpha-1-antiproteinase antibody
    • alpha1 proteinase inhibitor antibody
    • Alpha1AT antibody
    • Dom1 antibody
    • PI antibody
    • PI1 antibody
    • PRO2275 antibody
    • Serine (or cysteine) proteinase inhibitor clade A member 1 antibody
    • Serine protease inhibitor 1-1 antibody
    • Serine protease inhibitor A1a antibody
    • Serpin A1 antibody
    • Serpin A1a antibody
    • Serpin peptidase inhibitor clade A member 1 antibody
    • Serpina1 antibody
    • Short peptide from AAT antibody
    • SPAAT antibody
    • Spi1-1 antibody
    see all

Anti-alpha 1 Antitrypsin antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling alpha 1 Antitrypsin with ab9373.

  • ICC/IF image of ab9373 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9373, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab9373 staining alpha 1 Antitrypsin in formalin-fixed, paraffin-embedded Human tonsil tissue by Immunohistochemistry.

Anti-alpha 1 Antitrypsin antibody (ab9373)参考文献

This product has been referenced in:
  • Jacobo SM  et al. Age-related macular degeneration-associated silent polymorphisms in HtrA1 impair its ability to antagonize insulin-like growth factor 1. Mol Cell Biol 33:1976-90 (2013). Read more (PubMed: 23478260) »
  • Kamimura K  et al. Parameters Affecting Image-guided, Hydrodynamic Gene Delivery to Swine Liver. Mol Ther Nucleic Acids 2:e128 (2013). IHC-P ; Human . Read more (PubMed: 24129227) »

See all 3 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Tissue lysate - other (Synovial fluid)
Total protein in input 500 µg
Immuno-precipitation step Other - Agarose amine-reactive support
Specification Synovial fluid
Username

Dr. Tania Colasanti

Verified customer

提交于 Jun 03 2016

Application Western blot
Sample Human Tissue lysate - other (Synovial fluid)
Gel Running Conditions Reduced Denaturing (7% gel SDS PAGE)
Loading amount 2 µg
Specification Synovial fluid
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Tania Colasanti

Verified customer

提交于 May 23 2016

Application ELISA
Sample Human Purified protein (Native)
Specification Native
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C
Type Indirect
Username

Dr. Tania Colasanti

Verified customer

提交于 May 23 2016

Application ELISA
Sample Rabbit Purified protein (purified protein)
Specification purified protein
Type Sandwich (Capture)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.25% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Feb 12 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (liver)
Specification liver
Fixative Acetone
Permeabilization No
Blocking step GTx30972 as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Dec 21 2012

Thank you for your inquiry.

It is not possible to provide a exact concentration of this antibody.

ab9373 was purified using Protein A. This means that also other non Antitrypsin specific IgGs will be present.

The total pro...

Read More

The concentration is 400 ug/ml.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"