alpha 1 Antichymotrypsin overexpression 293T裂解液(全细胞) (ab94078)

概述

  • 产品名称alpha 1 Antichymotrypsin overexpression 293T裂解液(全细胞)
  • 常规说明ab94078 is a 293T cell transfected lysate in which Human alpha 1 Antichymotrypsin has been transiently over-expressed using a pCMV-alpha 1 Antichymotrypsin plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 背景Disease: Defects in SERPINA3 may be a cause of chronic obstructive pulmonary disease (COPD) [MIM:107280]. Domain: The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable. Function: Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2. Similarity: Belongs to the serpin family. Tissue specificity: Plasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains.

应用

Our Abpromise guarantee covers the use of ab94078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

alpha 1 Antichymotrypsin overexpression 293T lysate (whole cell) 图像

  • ab94078 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-alpha 1 Antichymotrypsin antibody (ab54693) at 1/500 dilution

    Lane 1 : alpha 1 Antichymotrypsin 293T Transfected Lysate - Positive Control (ab94078)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

alpha 1 Antichymotrypsin overexpression 293T lysate (whole cell) (ab94078)参考文献

ab94078 has not yet been referenced specifically in any publications.

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