概述

  • 产品名称Anti-alpha 1 Antichymotrypsin抗体
    参阅全部 alpha 1 Antichymotrypsin 一抗
  • 描述
    小鼠单克隆抗体to alpha 1 Antichymotrypsin
  • 经测试应用适用于: IHC-P, WB, Sandwich ELISAmore details
  • 种属反应性
    与反应: Recombinant Fragment
    预测可用于: Human, Gorilla, Dictyostelium discoideum
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 25-424 of Human alpha 1 Antichymotrypsin

性能

应用

Our Abpromise guarantee covers the use of ab54693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Sandwich ELISA Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to alpha 1 Antichymotrypsin (ab9374). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to alpha 1 Antichymotrypsin (ab9374) as Detection.

靶标

  • 功能Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.
  • 组织特异性Plasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains.
  • 疾病相关Defects in SERPINA3 may be a cause of chronic obstructive pulmonary disease (COPD) [MIM:107280].
  • 序列相似性Belongs to the serpin family.
  • 结构域The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • SERPINA3 antibody
    • AACT antibody
    • AACT_HUMAN antibody
    • ACT antibody
    • Alpha-1-antichymotrypsin His-Pro-less antibody
    • Antichymotrypsin antibody
    • Cell growth inhibiting gene 24/25 protein antibody
    • Cell growth-inhibiting gene 24/25 protein antibody
    • GIG24 antibody
    • GIG25 antibody
    • Growth inhibiting protein 24 antibody
    • Growth inhibiting protein 25 antibody
    • MGC88254 antibody
    • Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase, antitrypsin) member 3 antibody
    • Serine (or cysteine) proteinase inhibitor clade A member 3 antibody
    • Serine proteinase inhibitor clade A member 3 antibody
    • Serpin A3 antibody
    • Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 3 antibody
    • SERPINA3 antibody
    see all

Anti-alpha 1 Antichymotrypsin antibody 图像

  • Standard Curve for alpha 1 Antichymotrypsin (Analyte: alpha 1 Antichymotrypsin protein (Human) (ab80517) ); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal to alpha 1 Antichymotrypsin (ab54693) at 5µg/ml and Detector Antibody Rabbit polyclonal to alpha 1 Antichymotrypsin (ab9374) at 0.5µg/ml.
  • alpha 1 Antichymotrypsin antibody (ab54693) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human ovary, clear cell carcinoma.
  • Western blot against tagged recombinant protein immunogen using ab54693 alpha 1 Antichymotrypsin antibody at 1ug/ml. Predicted band size of immunogen is 70 kDa.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

实验方案

Anti-alpha 1 Antichymotrypsin antibody (ab54693)参考文献

This product has been referenced in:
  • Sohn EH  et al. Structural and biochemical analyses of choroidal thickness in human donor eyes. Invest Ophthalmol Vis Sci 55:1352-60 (2014). WB ; Human . Read more (PubMed: 24519422) »

See 1 Publication for this product

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