Anti-alpha 1 Adrenergic Receptor抗体(ab3462)

概述

  • 产品名称
    Anti-alpha 1 Adrenergic Receptor抗体
    参阅全部 alpha 1 Adrenergic Receptor 一抗
  • 描述
    兔多克隆抗体to alpha 1 Adrenergic Receptor
  • 特异性
    By Western blot, this antibody detects a 60 kDa protein representing the A1AR from mouse kidney membrane preparations, mouse liver, HeLa cell lysates, and zebra fish samples. Immunofluorescence staining of A1AR in mouse kidney distal tubule cells results in staining of the plasma membrane.
  • 经测试应用
    适用于: WB, ELISA, ICC, ICC/IF, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Cat, Human, Drosophila melanogaster, Zebrafish
    预测可用于: Rabbit, Guinea pig, Hamster, Cow, Dog, Pig, Japanese ricefish
  • 免疫原

    Synthetic peptide corresponding to Human alpha 1 Adrenergic Receptor aa 339-349 (intracellular). Immunizing peptide corresponds to residues in the 3rd intracellular loop of human A1AR. Thiis sequence is 100% conserved in all A1AR subtypes examined.
    Sequence:

    KFSREKKAAKT


    (Peptide available as ab41797)

  • 阳性对照
    • mouse kidney membrane preparations, mouse liver lysate, HeLa cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab3462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/400. Detects a band of approximately 60 kDa.Can be blocked with alpha 1 Adrenergic Receptor peptide (ab41797).
ELISA Use at an assay dependent concentration.
ICC 1/10 - 1/1000.
ICC/IF 1/1000.
IP Use at an assay dependent concentration.

use 5 ug/mg lysate

IHC-P 1/50 - 1/200.

靶标

图片

  • Immunocytochemistry/Immunofluorescence analysis of alpha 1 Adrenergic Receptor (green) showing staining in the cytoplasm of HepG2 cells (right) compared to a negative control (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3462 in 3% BSA-PBS at a dilution of 1:100 overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • ab3462 labelling alpha 1 Adrenergic Receptor in the cytoplasm and membrane of Human prostate tissue (right) compared with a negative control (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated in the primary antibody (1:100 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunocytochemistry/Immunofluorescence analysis of alpha 1 Adrenergic Receptor (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3462 in 3% BSA-PBS at a dilution of 1:100 overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • ab3462 labelling alpha 1 Adrenergic Receptor in the cytoplasm and membrane of Mouse brain tissue (right) compared with a negative control (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated in the primary antibody (1:100 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunocytochemistry/Immunofluorescence analysis of alpha 1 Adrenergic Receptor (green) showing staining in the cytoplasm of PC-3 cells (right) compared to a negative control (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3462 in 3% BSA-PBS at a dilution of 1:100 overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • Immunolocalization of alpha 1 Adrenergic Receptor in mouse distal convoluted tubule using ab3462.

文献

This product has been referenced in:
  • Sumi-Ichinose C  et al. Sepiapterin reductase gene-disrupted mice suffer from hypertension with fluctuation and bradycardia. Physiol Rep 5:N/A (2017). Read more (PubMed: 28320892) »
  • de França SA  et al. A Low-Protein, High-Carbohydrate Diet Stimulates Thermogenesis in the Brown Adipose Tissue of Rats via ATF-2. Lipids 51:303-10 (2016). Read more (PubMed: 26781764) »

See all 8 Publications for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
Yes - 0.2% Triton X-100
Specification
heart
Blocking step
Serum as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 01 2016

Application
Western blot
Sample
Mouse Tissue lysate - other (cellular fractionation of hindpaw skin protein)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
cellular fractionation of hindpaw skin protein
Blocking step
LiCOR blocking buffer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Sep 30 2015

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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Thank you for contacting Abcam.

For the antibody ab3462, I was able to locate WB conditions that were used for testing. Please see below.

The samples tested were HeLa cell lysate. rat liver, mouse liver, drosophila and zebra fish. ...

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I am sorry that you have experienced this error! I would be happy to issue a free of charge replacement of the vial of ab3462 you received that did not contain the full volume. This is a mistake on our part, and I certainly want to ensure that you rece...

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Thank you for your inquiry.

I do not suggest to dilute the antibody in the same buffer it is stored in.
The buffer to create the working solutions should be according to the application the antibody is used.

I cannot recommen...

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We believe in providing our customers with all the available information about a product whether that information is positive or negative. However, we do stand behind the quality of this antibody and guarant...

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I am beginning to gather the CoC's you have requested. When you have a chance, please provide the lot number from each catalog number so I can include this information on the documentation. Thanks for the additional information.

Thank you for contacting us. I have received more detailed information form the lab: The western blot procedure is as following: Run a SDS-PAGE gel and transfer to membrane. Block in 5% non-fat dry milk in TBS buffer for 1h at RT. Wash with TBS...

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Yes, you are right, I double checked the sequence and there is no alpha 1 adrenergic receptor in Drosophila. This was based on WB results as similar size protein was obtained. We have removed Dm and apologize for the confusion. I did a Blast sear...

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