Anti-Alpha 1 Acid Glycoprotein抗体(ab52691)

概述

  • 产品名称Anti-Alpha 1 Acid Glycoprotein抗体
    参阅全部 Alpha 1 Acid Glycoprotein 一抗
  • 描述
    小鼠多克隆抗体to Alpha 1 Acid Glycoprotein
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide: ENGTISRYVG GQEHFAHLLI LRDTKTYMLA FDVNDEKNWG LSVYADKPET TKEQLGEFYE ALDCLRIPKS DVVYTDWKKD KCEPLEKQHE KERKQEEGES , corresponding to amino acids 102-202 of Human Alpha 1 Acid Glycoprotein

  • 常规说明


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 50% Glycerol, Whole serum
  • 纯度Whole antiserum
  • Primary antibody说明This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab52691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 24 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

靶标

  • 功能Appears to function in modulating the activity of the immune system during the acute-phase reaction.
  • 组织特异性Expressed by the liver and secreted in plasma.
  • 序列相似性Belongs to the calycin superfamily. Lipocalin family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A1AG1_HUMAN antibody
    • AGP 1 antibody
    • AGP A antibody
    • AGP antibody
    • AGP1 antibody
    • Alpha 1 acid glycoprotein antibody
    • Alpha-1-acid glycoprotein 1 antibody
    • Epididymis secretory sperm binding protein Li 153w antibody
    • HEL S 153w antibody
    • OMD 1 antibody
    • ORM antibody
    • ORM1 antibody
    • Orosomucoid 1 antibody
    • Orosomucoid-1 antibody
    see all

Anti-Alpha 1 Acid Glycoprotein antibody 图像

  • All lanes : Anti-Alpha 1 Acid Glycoprotein antibody (ab52691) at 1/1000 dilution

    Lane 1 : Left: The negative control lane of ~20ug of a total protein extract from E coli with ~50ng to 100 ng of a fusion protein of an irrelevant antigen.
    Lane 2 : Right: test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (antigen fusion protein).

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

    Predicted band size : 24 kDa

Anti-Alpha 1 Acid Glycoprotein antibody (ab52691)参考文献

ab52691 has not yet been referenced specifically in any publications.

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