Application
Western blot
Sample
Rat Cell lysate - whole cell (Lung)
Gel Running Conditions
Reduced Denaturing
Loading amount
10000 cells
Specification
Lung
Blocking step
(agent) for 30 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Diluent: Blocking Solution Casein
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Chromoenic Stain Western Breeze Rabbit anti mouse
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Detection
Detection method
Chromogenic
Exposure
15 minute(s) and 0 second(s)
Bands
Specific: 170 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Oct 08 2015