Application
Western blot
Sample
Rat Cell lysate - whole cell (cortex)
Gel Running Conditions
Reduced Denaturing (7%)
Loading amount
10 µg
Specification
cortex
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA, 0.1% Tween 20 in PBS
Dilution
1/500
Secondary antibody
Dilution
1/8000
Name
Non-Abcam antibody was used: anti-mouse antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 second(s)
Bands
Specific: 100 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Mar 21 2016