Application
Western blot
Sample
Dog Cell lysate - other (canine dermal fibroblasts-membrane fraction)
Gel Running Conditions
Reduced Denaturing (7.5%)
Loading amount
10 µg
Specification
canine dermal fibroblasts-membrane fraction
Blocking step
Block ace as blocking agent for 50 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: anti-mouse IgG goat antibody
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
20 minute(s) and 0 second(s)
Bands
Specific: 90 kDa Non-specific: 140 kDa
Additional data
Abcam response
To improve the intensity of the band at 90 kDa, we recommend to load more proteins such as 50 µg. Changing the blocking reagent can help to decrease the general background. We recommend using 5% BSA as the blocking agent.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Mar 11 2016