Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Mouse Tissue sections (14 days old embryonic brain section, 16 micron)
Specification
14 days old embryonic brain section, 16 micron
Permeabilization
Yes - Triton X100
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS with 0.1% Triton X100, 1% serum
Dilution
1/300
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 594 Donkey Anti-Mouse IgG (H+L) Antib
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/700
Additional data
Additional Notes
Sections underwent antigen-retrieval in citrate buffer, pH 6, at 97 degree for 20 min prior to staining. Signal was largely derived from choroid plexus as shown in the images.
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提交于 Jan 23 2015