Anti-alpha 1 Fetoprotein抗体(ab46799)
Key features and details
- Rabbit polyclonal to alpha 1 Fetoprotein
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Cat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-alpha 1 Fetoprotein抗体
参阅全部 alpha 1 Fetoprotein 一抗 -
描述
兔多克隆抗体to alpha 1 Fetoprotein -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Mouse, Rat, Cat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HepG2 cell lysate; Human fetal liver tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab46799于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P | (4) |
Use at an assay dependent concentration.
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 69 kDa).
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说明 |
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IHC-P
Use at an assay dependent concentration. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 69 kDa). |
靶标
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功能
Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. -
组织特异性
Plasma. Synthesized by the fetal liver and yolk sac. -
序列相似性
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
发展阶段
Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma. -
翻译后修饰
Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
Sulfated. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 174 Human
- Entrez Gene: 11576 Mouse
- Entrez Gene: 24177 Rat
- Omim: 104150 Human
- SwissProt: P02771 Human
- SwissProt: P02772 Mouse
- SwissProt: P02773 Rat
- Unigene: 518808 Human
see all -
别名
- Afp antibody
- AFPD antibody
- Alpha fetoglobulin antibody
see all
图片
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All lanes : Anti-alpha 1 Fetoprotein antibody (ab46799) at 1 µg/ml
Lane 1 : HepG2 (Human) Whole Cell Lysate
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 73 kDa why is the actual band size different from the predicted?
Additional bands at: 58 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minuteThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab46799 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
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Anti-alpha 1 Fetoprotein antibody (ab46799) at 1/50000 dilution + HepG2 cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP labeled, at 1/2000 dilution
Predicted band size: 69 kDa
Observed band size: 69 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (38)
ab46799 被引用在 38 文献中.
- Kim W et al. Suppression of transient receptor potential melastatin 7 regulates pluripotency, proliferation, and differentiation of mouse embryonic stem cells via mechanistic target of rapamycin-extracellular signal-regulated kinase activation. J Cell Biochem 123:547-567 (2022). PubMed: 34958137
- Kopasz AG et al. A versatile transposon-based technology to generate loss- and gain-of-function phenotypes in the mouse liver. BMC Biol 20:74 (2022). PubMed: 35361222
- Aktas RG et al. Long-Term Characteristics of Human-Derived Biliary Organoids under a Single Continuous Culture Condition. Cells 11:N/A (2022). PubMed: 36497057
- Ramos IPR et al. Granulocyte Colony-Stimulating Factor Treatment Before Radiotherapy Protects Against Radiation-Induced Liver Disease in Mice. Front Pharmacol 12:725084 (2021). PubMed: 34867327
- Men H et al. Early-Life Exposure to Low-Dose Cadmium Accelerates Diethylnitrosamine and Diet-Induced Liver Cancer. Oxid Med Cell Longev 2021:1427787 (2021). PubMed: 34876963