Application
Immunoprecipitation
Sample
Human Tissue lysate - other (Synovial fluid)
Total protein in input
500 µg
Immuno-precipitation step
Other - Agarose amine-reactive support
Specification
Synovial fluid
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/50
Additional data
Additional Notes
Checking all the steps of the IP protocol in WB, ab9373 antibody is detected in the antibody coupling flow-through and with the eluted antigen, so it doesn't completely couple to the resin (I also used less amount of ab9373, obtaining the same result)
Abcam response
Thank you for taking the time to submit this review. We have not yet tested this antibody in IP. Your data suggests that it may not be suitable for use in this application. We welcome more feedback from other researchers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Tania Colasanti
Verified customer
提交于 Jun 03 2016