Application
Western blot
Sample
Human Tissue lysate - other (Synovial fluid)
Gel Running Conditions
Reduced Denaturing (7% gel SDS PAGE)
Loading amount
2 µg
Specification
Synovial fluid
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS 0,05% tween20, 5% non-fat dry milk
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: Blotting Grade Goat Anti-Rabbit IgG (H+L) HRP Conj
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 52 kDa Non-specific: 50 kDa
Positive control
Human alpha 1 antitrypsin full-length protein ab167788
Additional data
Additional Notes
ab9373 antibody was tested against the synovial fluid (lane 1 of the figure) and the ab167788 protein (lane 2).
Nitrocellulose membrane was used for WB (transfer conditions: 350mA, 1h 30min, 4°C)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Tania Colasanti
Verified customer
提交于 May 23 2016