Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 1mM EDTA 10mM Tris-HCl (pH=9.0)
Permeabilization
No
Specification
Liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TBS +0.1% Tween 20
Dilution
1/2000
Secondary antibody
Dilution
1/300
Name
Non-Abcam antibody was used: Rabbit anti-Mouse IgG antibody HRP conjugate
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Does not detected with rat.
Abcam response
This antibody is yet to be tested with rat samples, therefore we do not have any supporting data. We would recommend staining human tissue sections alongside as positive control.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Feb 26 2016