概述

  • 产品名称
    Anti-ALP抗体
    参阅全部 ALP 一抗
  • 描述
    兔多克隆抗体to ALP
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (PQTVILPGPA PWGFRLSGGI DFNQPLVITR ITPGSKAAAA NLCPGDVILA) of human ALP (NP_055291).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照
    • Fetal liver lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified by peptide affinity chromatography method.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab83259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/1562500.

靶标

图片

  • Western blot - Anti-ALP antibody (ab83259)
    Western blot - Anti-ALP antibody (ab83259)
    Anti-ALP antibody (ab83259) at 1 µg/ml (5% skim milk / PBS buffer) + fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 39 kDa
    Observed band size: 40 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.



    Gel concentration: 12%

实验方案

文献

This product has been referenced in:
  • Riedl I  et al. Association of the ACTN3 R577X polymorphism with glucose tolerance and gene expression of sarcomeric proteins in human skeletal muscle. Physiol Rep 3:N/A (2015). Read more (PubMed: 25780092) »

See 1 Publication for this product

发表研究结果有使用 ab83259？请让我们知道，以便我们可以引用本数据表中的参考文章。

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