- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
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产品名称Anti-ALP抗体
参阅全部 ALP 一抗 -
描述兔多克隆抗体to ALP
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经测试应用适用于: WB, ELISAmore details
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种属反应性与反应: Human
预测可用于: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
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免疫原
Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (PQTVILPGPA PWGFRLSGGI DFNQPLVITR ITPGSKAAAA NLCPGDVILA) of human ALP (NP_055291).
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阳性对照
- Fetal liver lysate.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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存储溶液Preservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
纯度Immunogen affinity purified
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纯化说明Purified by peptide affinity chromatography method.
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab83259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. | |
| ELISA | Use at an assay dependent concentration. ELISA titre using peptide based assay: 1/1562500. |
靶标
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功能May play a role in the organization of actin filament arrays within muscle cells.
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组织特异性Isoform 1 is highly expressed in differentiated skeletal muscle. Isoform 2 is heart-specific.
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序列相似性Contains 1 LIM zinc-binding domain.
Contains 1 PDZ (DHR) domain. -
细胞定位Cytoplasm > myofibril > sarcomere > Z line. Localizes to myofiber Z-lines.
- Information by UniProt
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数据库链接
- Entrez Gene: 538516 Cow
- Entrez Gene: 27295 Human
- Entrez Gene: 53318 Mouse
- Entrez Gene: 414421 Pig
- Entrez Gene: 114108 Rat
- Omim: 605889 Human
- SwissProt: Q3SYZ8 Cow
- SwissProt: Q53GG5 Human
see all -
别名
- Actinin associated LIM protein antibody
- Actinin-associated LIM protein antibody
- Alpha actinin 2 associated LIM protein antibody
see all
Anti-ALP antibody 图像
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Western blot - ALP antibody (ab83259)Anti-ALP antibody (ab83259) at 1 µg/ml (5% skim milk / PBS buffer) + fetal liver lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 39 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
Anti-ALP antibody (ab83259)参考文献
This product has been referenced in:
- Riedl I et al. Association of the ACTN3 R577X polymorphism with glucose tolerance and gene expression of sarcomeric proteins in human skeletal muscle. Physiol Rep 3:N/A (2015). Read more (PubMed: 25780092) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"