产品名称 Anti-ALP抗体 参阅全部 ALP 一抗
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Full length human protein (AAH27870, 1 a.a. ~ 317 a.a)
ALP transfected 293T cell lysate.
存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
存储溶液 Preservative: None
Constituents: 1X PBS, pH 7.2
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纯度 Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 39 kDa).
功能 May play a role in the organization of actin filament arrays within muscle cells.
组织特异性 Isoform 1 is highly expressed in differentiated skeletal muscle. Isoform 2 is heart-specific.
序列相似性 Contains 1 LIM zinc-binding domain. Contains 1 PDZ (DHR) domain.
细胞定位 Cytoplasm > myofibril > sarcomere > Z line. Localizes to myofiber Z-lines.
Information by UniProt
Actinin associated LIM protein antibody
Actinin-associated LIM protein antibody
Alpha actinin 2 associated LIM protein antibody
Anti-ALP antibody 图像
Western blot - ALP antibody (ab67228)
All lanes : Anti-ALP antibody (ab67228) at 1/500 dilution Lane 1 : ALP transfected 293T cell lysate Lane 2 : Non-transfected 293T cell lysate Lysates/proteins at 25 µg per lane. Secondary Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution Predicted band size : 39 kDa Observed band size : 35 kDa ( why is the actual band size different from the predicted?)
Anti-ALP antibody (ab67228)参考文献
This product has been referenced in:
et al. Disruption of kif3a results in defective osteoblastic differentiation in dental mesenchymal stem/precursor cells via the Wnt signaling pathway. Mol Med Rep 14:1891-900 (2016).
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