Anti-ALKBH1抗体[hABH1-151] (ab49738)

概述

  • 产品名称Anti-ALKBH1抗体[hABH1-151]
    参阅全部 ALKBH1 一抗
  • 描述
    小鼠单克隆抗体[hABH1-151] to ALKBH1
  • 特异性This antibody does not recognize hABH2 or hABH3.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human, Monkey
  • 免疫原

    Synthetic peptide corresponding to Human ALKBH1 aa 19-34 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    GE DAFRKLFRFY RQSR

  • 阳性对照
    • A total cell extract of 293T cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 克隆单克隆
  • 克隆编号hABH1-151
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab49738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 43 kDa.

靶标

  • 功能Dioxygenase that repairs alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation. Requires molecular oxygen, alpha-ketoglutarate and iron. May have a role in placental trophoblast lineage differentiation (By similarity). Has DNA lyase activity and introduces double-stranded breaks at abasic sites. Cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites. DNA lyase activity does not require alpha-ketoglutarate and iron.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the alkB family.
    Contains 1 Fe2OG dioxygenase domain.
  • 细胞定位Mitochondrion. Nucleus. Mainly localizes in euchromatin, largely excluded from heterochromatin and nucleoli.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ABH antibody
    • ABH1 antibody
    • alkB antibody
    • alkB homolog 1 histone H2A dioxygenase antibody
    • AlkB, alkylation repair homolog 1 (E. coli) antibody
    • ALKB1_HUMAN antibody
    • ALKBH antibody
    • ALKBH1 antibody
    • Alkylated DNA repair protein alkB homolog 1 antibody
    • Alkylation repair homolog 1 antibody
    • Alkylation repair, alkB homolog antibody
    • Alpha-ketoglutarate-dependent dioxygenase ABH1 antibody
    • DNA lyase ABH1 antibody
    • DNA oxidative demethylase ALKBH1 antibody
    • hABH antibody
    see all

Anti-ALKBH1 antibody [hABH1-151] (ab49738)参考文献

ab49738 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20000 cells
Gel Running Conditions Reduced Denaturing (12.5%)
Sample Rat Tissue lysate - whole (heart, brain, muscle)
Specification heart, brain, muscle
Blocking step Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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提交于 Dec 06 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"