Anti-Alkaline Phosphatase,组织Non-Specific抗体(ab97384)

概述

  • 产品名称
    Anti-Alkaline Phosphatase,组织Non-Specific抗体
    参阅全部 Alkaline Phosphatase, Tissue Non-Specific 一抗
  • 描述
    兔多克隆抗体to Alkaline Phosphatase,组织Non-Specific
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Cat
  • 免疫原

    Recombinant fragment containing a sequence corresponding to a region within amino acids 1 and 369 of Human Alkaline Phosphatase, Tissue Non-Specific (NP_000469).

  • 阳性对照
    • Hela, HepG2, MOLT4 and Raji cell lines; OVCAR3 xenograft

性能

应用

Our Abpromise guarantee covers the use of ab97384 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 57 kDa.
IHC-P 1/100 - 1/500.

靶标

图片

  • Anti-Alkaline Phosphatase, Tissue Non-Specific antibody (ab97384) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 57 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of Alkaline Phosphatase, Tissue Non-Specific in paraffin embedded OVCAR3 xenograft, using ab97384 at a 1/100 dilution.

文献

This product has been referenced in:
  • Ren T  et al. CD49b inhibits osteogenic differentiation and plays an important role in osteosarcoma progression. Oncotarget 8:87848-87859 (2017). Read more (PubMed: 29152125) »

See 1 Publication for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (skin)
Specification
skin
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 15µg/mL · Temperature: 25°C
Username

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Verified customer

提交于 Apr 11 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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