Anti-Alkaline Phosphatase,组织Non-Specific抗体(ab65834)
Key features and details
- Rabbit polyclonal to Alkaline Phosphatase, Tissue Non-Specific
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Alkaline Phosphatase,组织Non-Specific抗体
参阅全部 Alkaline Phosphatase, Tissue Non-Specific 一抗 -
描述
兔多克隆抗体to Alkaline Phosphatase,组织Non-Specific -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Rat, Human -
免疫原
Synthetic peptide corresponding to Human Alkaline Phosphatase, Tissue Non-Specific aa 1-100 (N terminal).
Database link: P05186 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservatives: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 0.1% Dibasic monohydrogen sodium phosphate, 2.5% BSA, 0.45% Sodium chloride -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab65834于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
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IHC-P |
Use at an assay dependent concentration.
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说明 |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa). |
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
This isozyme may play a role in skeletal mineralization. -
疾病相关
Hypophosphatasia
Hypophosphatasia childhood type
Hypophosphatasia infantile type -
序列相似性
Belongs to the alkaline phosphatase family. -
翻译后修饰
N-glycosylated. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 249 Human
- Entrez Gene: 25586 Rat
- Omim: 171760 Human
- SwissProt: P05186 Human
- SwissProt: P08289 Rat
- Unigene: 75431 Human
- Unigene: 82764 Rat
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别名
- AKP2 antibody
- Alkaline phosphatase liver/bone/kidney antibody
- Alkaline phosphatase liver/bone/kidney isozyme antibody
see all
图片
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All lanes : Anti-Alkaline Phosphatase, Tissue Non-Specific antibody (ab65834) at 0.5 µg/ml
Lane 1 : Human Placenta Tissue Lysate at 50 µg
Lanes 2-3 : JURKAT Whole Cell Lysate at 40 µg
Predicted band size: 57 kDa -
This image shows ab65834 at 1-2µg/ml staining cytoplasmic Alkaline Phosphatase in rat small intestine tissue.
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Anti-Alkaline Phosphatase, Tissue Non-Specific antibody (ab65834) at 2 µg/ml + HeLa cell lysate
Predicted band size: 57 kDa
Observed band size: 57 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (33)
ab65834 被引用在 33 文献中.
- Zhang Y et al. Applying exercise-mimetic engineered skeletal muscle model to interrogate the adaptive response of irisin to mechanical force. iScience 25:104135 (2022). PubMed: 35434556
- Sun X et al. Paeoniflorin Ameliorates Hyperprolactinemia-Induced Inhibition of Osteoblastogenesis by Suppressing the NF-κB Signaling Pathway. Int J Endocrinol 2022:4572033 (2022). PubMed: 35465073
- Yan C et al. Extracellular vesicles from the inflammatory microenvironment regulate the osteogenic and odontogenic differentiation of periodontal ligament stem cells by miR-758-5p/LMBR1/BMP2/4 axis. J Transl Med 20:208 (2022). PubMed: 35562763
- Wu M et al. JAM-A facilitates hair follicle regeneration in alopecia areata through functioning as ceRNA to protect VCAN expression in dermal papilla cells. Precis Clin Med 5:pbac020 (2022). PubMed: 36132055
- Wang Q et al. miR-153-3p inhibits osteogenic differentiation of BMSCs by down-regulating the expression of RUNX2 in a high glucose environment. Am J Transl Res 14:7027-7039 (2022). PubMed: 36398274