Anti-Alkaline Phosphatase,组织Non-Specific抗体(ab65834)

概述

  • 产品名称Anti-Alkaline Phosphatase,组织Non-Specific抗体
    参阅全部 Alkaline Phosphatase, Tissue Non-Specific 一抗
  • 描述
    兔多克隆抗体to Alkaline Phosphatase,组织Non-Specific
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide from the N-terminus of Human Alkaline Phosphatase

  • 阳性对照
    • HeLa cells. Small intestine.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab65834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 20634292
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
IHC-P Use at an assay dependent concentration.

靶标

  • 功能This isozyme may play a role in skeletal mineralization.
  • 疾病相关Hypophosphatasia
    Hypophosphatasia childhood type
    Hypophosphatasia infantile type
  • 序列相似性Belongs to the alkaline phosphatase family.
  • 翻译后修饰N-glycosylated.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AKP2 antibody
    • Alkaline phosphatase liver/bone/kidney antibody
    • Alkaline phosphatase liver/bone/kidney isozyme antibody
    • Alkaline phosphatase tissue nonspecific isozyme antibody
    • Alkaline phosphatase, tissue-nonspecific isozyme antibody
    • Alkaline phosphomonoesterase antibody
    • Alpl antibody
    • AP TNAP antibody
    • AP-TNAP antibody
    • APTNAP antibody
    • BAP antibody
    • FLJ40094 antibody
    • FLJ93059 antibody
    • Glycerophosphatase antibody
    • HOPS antibody
    • Liver/bone/kidney type alkaline phosphatase antibody
    • MGC161443 antibody
    • MGC167935 antibody
    • PHOA antibody
    • PPBT_HUMAN antibody
    • Tissue non specific alkaline phosphatase antibody
    • Tissue nonspecific ALP antibody
    • TNAP antibody
    • TNSALP antibody
    see all

Anti-Alkaline Phosphatase, Tissue Non-Specific antibody 图像

  • This image shows ab65834 at 1-2µg/ml staining cytoplasmic Alkaline Phosphatase in rat small intestine tissue.
  • Anti-Alkaline Phosphatase, Tissue Non-Specific antibody (ab65834) at 2 µg/ml + HeLa cell lysate

    Predicted band size : 57 kDa
    Observed band size : 57 kDa
  • ICC/IF image of ab65834 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65834, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Alkaline Phosphatase, Tissue Non-Specific antibody (ab65834)参考文献

This product has been referenced in:
  • Davis H  et al. Aberrant epithelial GREM1 expression initiates colonic tumorigenesis from cells outside the stem cell niche. Nat Med 21:62-70 (2015). Read more (PubMed: 25419707) »
  • Davis H  et al. Investigation of the atypical FBXW7 mutation spectrum in human tumours by conditional expression of a heterozygous propellor tip missense allele in the mouse intestines. Gut N/A:N/A (2013). IHC-P ; Mouse . Read more (PubMed: 23676439) »

See all 4 Publications for this product

Product Wall

Thank you for contacting Abcam.
Although the exact immnuogen sequences are considered to be proprietary, I can let you the following information narrowing down the regions that these antibodies recognize:
- ab108337 - Between ***
- ab658...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this ha...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Kidney)
Specification Kidney
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH6/8
Permeabilization No
Blocking step Serum Free Protein Block as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 21°C
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提交于 Jun 13 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"