Anti-Alkaline Phosphatase,组织Non-Specific抗体[2F4] (ab126820)
Key features and details
- Mouse monoclonal [2F4] to Alkaline Phosphatase, Tissue Non-Specific
- Suitable for: WB, ELISA, IHC-P, Flow Cyt, ICC/IF
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Alkaline Phosphatase,组织Non-Specific抗体[2F4]
参阅全部 Alkaline Phosphatase, Tissue Non-Specific 一抗 -
描述
小鼠单克隆抗体[2F4] to Alkaline Phosphatase,组织Non-Specific -
宿主
Mouse -
经测试应用
适用于: WB, ELISA, IHC-P, Flow Cyt, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human Alkaline Phosphatase, Tissue Non-Specific. expressed in E.coli
Database link: P05186 -
阳性对照
- Recombinant Human ALPL protein (AA: 18-502), HeLa and NTERA-2 cell lysates. This antibody gave a positive result when used in the following formaldehyde/methanol fixed cell lines: HepG2
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常规说明
This product was changed from ascites to supernatant. Lot no’s high than GR307490-5 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20ºC. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purified from tissue culture supernatant. -
克隆
单克隆 -
克隆编号
2F4 -
同种型
IgG1 -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab126820于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/2000. Detects a band of approximately 57.3, 78.9 kDa (predicted molecular weight: 57, 78.9 kDa).
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ELISA |
1/10000.
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IHC-P |
1/200 - 1/1000.
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Flow Cyt |
1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
1/100.
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说明 |
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WB
1/500 - 1/2000. Detects a band of approximately 57.3, 78.9 kDa (predicted molecular weight: 57, 78.9 kDa). |
ELISA
1/10000. |
IHC-P
1/200 - 1/1000. |
Flow Cyt
1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/100. |
靶标
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功能
This isozyme may play a role in skeletal mineralization. -
疾病相关
Hypophosphatasia
Hypophosphatasia childhood type
Hypophosphatasia infantile type -
序列相似性
Belongs to the alkaline phosphatase family. -
翻译后修饰
N-glycosylated. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 249 Human
- Omim: 171760 Human
- SwissProt: P05186 Human
- Unigene: 75431 Human
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别名
- AKP2 antibody
- Alkaline phosphatase liver/bone/kidney antibody
- Alkaline phosphatase liver/bone/kidney isozyme antibody
see all
图片
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All lanes : Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [2F4] (ab126820) at 1/500 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : ALPL knockout HAP1 whole cell lysate
Lane 3 : Saos2 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 57, 78.9 kDaLanes 1 - 3: Merged signal (red and green). Green - ab126820 observed at 80 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab126820 was shown to specifically react with ALPL in wild-type HAP1 cells as signal was lost in ALPL knockout cells. Wild-type and ALPL knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab126820 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [2F4] (ab126820) at 1/500 dilution + Recombinant Human protein ALPL (AA: 18-502)
Predicted band size: 57, 78.9 kDa -
ICC/IF image of ab126820 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab126820 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in methanol fixed (100%, 5min) hepG2 cells used at 1/100 dilution.
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All lanes : Anti-Alkaline Phosphatase, Tissue Non-Specific antibody [2F4] (ab126820) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : NTERA-2 cell lysate
Predicted band size: 57, 78.9 kDa -
Example of standard curves obtained using ab126820. Control antigen 100ng (black), antigen at 10ng (purple), antigen at 50ng (blue), antigen at 100ng (red)
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Flow cytometric analysis of MCF-7 cells using ab126820 at 1/200 (green) and negative control (red).
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ab126820, at 1/200, staining Alkaline Phosphatase, Tissue Non-Specific in paraffin embedded Human ovarian cancer tissues by Immunohistochemistry with DAB staining.
实验方案
数据表及文件
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Datasheet download
文献 (7)
ab126820 被引用在 7 文献中.
- Huang X et al. Mechanical Sensing Element PDLIM5 Promotes Osteogenesis of Human Fibroblasts by Affecting the Activity of Microfilaments. Biomolecules 11:N/A (2021). PubMed: 34069539
- Peng Y et al. Regulation of the integrin αVβ3- actin filaments axis in early osteogenesis of human fibroblasts under cyclic tensile stress. Stem Cell Res Ther 12:523 (2021). PubMed: 34620239
- Khan AU et al. Lamin A/C-Dependent Translocation of Megakaryoblastic Leukemia-1 and β-Catenin in Cyclic Strain-Induced Osteogenesis. Cells 10:N/A (2021). PubMed: 34944031
- Kim H et al. A novel 3D indirect co-culture system based on a collagen hydrogel scaffold for enhancing the osteogenesis of stem cells. J Mater Chem B 8:9481-9491 (2020). PubMed: 32996551
- Bertram KM et al. Identification of HIV transmitting CD11c+ human epidermal dendritic cells. Nat Commun 10:2759 (2019). PubMed: 31227717
- Casanellas I et al. Matrix Nanopatterning Regulates Mesenchymal Differentiation through Focal Adhesion Size and Distribution According to Cell Fate. Biomimetics (Basel) 4:N/A (2019). PubMed: 31242712
- Parlato M et al. Human ALPI deficiency causes inflammatory bowel disease and highlights a key mechanism of gut homeostasis. EMBO Mol Med 10:N/A (2018). PubMed: 29567797