Anti-Alkaline Phosphatase抗体(ab75699)

概述

  • 产品名称Anti-Alkaline Phosphatase抗体
    参阅全部 Alkaline Phosphatase 一抗
  • 描述
    兔多克隆抗体to Alkaline Phosphatase
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A KLH conjugated synthetic peptide selected from the center region of human Alkaline Phosphatase.

  • 阳性对照
    • This antibody gave a positive signal in human liver tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab75699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 58 kDa).
ELISA 1/1000.
IHC-P 1/10 - 1/50.

靶标

  • 序列相似性Belongs to the alkaline phosphatase family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alkaline phosphatase, germ cell antibody
    • Alkaline phosphatase, testicular and thymus antibody
    • Akp2 antibody
    • Alkaline phosphatase intestinal antibody
    • Alkaline phosphatase intestinal precursor antibody
    • Alkaline phosphatase liver/bone/kidney antibody
    • Alkaline phosphatase placental antibody
    • Alkaline phosphatase placental like 2 antibody
    • Alkaline phosphatase placental like antibody
    • Alkaline phosphatase placental type antibody
    • Alkaline phosphatase, liver antibody
    • Alkaline phosphatase, tissue-nonspecific antibody
    • Alkaline phosphomonoesterase antibody
    • ALP 1 antibody
    • ALP antibody
    • ALP I antibody
    • ALP L antibody
    • ALP P antibody
    • ALPG antibody
    • Alpi antibody
    • ALPL antibody
    • ALPP antibody
    • ALPPL antibody
    • ALPPL2 antibody
    • AP TNAP antibody
    • FLJ40094 antibody
    • GCAP antibody
    • Germ cell alkaline phosphatase antibody
    • Glycerophosphatase antibody
    • HOPS antibody
    • IAP antibody
    • Intestinal alkaline phosphatase antibody
    • Intestinal-type alkaline phosphatase antibody
    • Kasahara isozyme antibody
    • Nagao isozyme antibody
    • OTTHUMP00000164357 antibody
    • Placental alkaline phosphatase antibody
    • Placental like alkaline phosphatase antibody
    • PLAP 1 antibody
    • PLAP antibody
    • PLAP like antibody
    • PPBI_HUMAN antibody
    • Regan isozyme antibody
    • Testicular and thymus alkaline phosphatase antibody
    • Tissue non specific alkaline phosphatase antibody
    • Tissue nonspecific ALP antibody
    • TNAP antibody
    • TNSALP antibody
    see all

Anti-Alkaline Phosphatase antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis human hepatocarcinoma tissue sections labeling Alkaline Phosphatase with ab75699 at 1/25. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 37°C. Heat mediated antigen retrieval was performed using a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A Peroxidase-conjugated Goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

  • Anti-Alkaline Phosphatase antibody (ab75699) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 58 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes
    Alkaline Phosphatase protein contains a 22-amino acid signal peptide and a 29-amino acid propeptide, both of which are removed in the mature form (SwissProt). This might explain the migration of the band at a lower molecular weight than predicted.

Anti-Alkaline Phosphatase antibody (ab75699)参考文献

This product has been referenced in:
  • Cerwinka WH  et al. Differentiation of human mesenchymal stem cell spheroids under microgravity conditions Cell Regeneration 1: (2012). IHC-P ; Human . Read more (PubMed: ) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Bone)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
Permeabilization No
Specification Bone
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative 10% buffered formalin
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Abcam user community

Verified customer

提交于 Feb 11 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (skin tumor)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH6
Permeabilization No
Specification skin tumor
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative 10% buffered formalin
Username

Abcam user community

Verified customer

提交于 Oct 10 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"