概述

  • 产品名称Anti-ALK抗体[SP8]
    参阅全部 ALK 一抗
  • 描述
    兔单克隆抗体[SP8] to ALK
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Recombinant fragment: WQHQP EDRP NFAIIL ERI EYCTQDP DV INTALPIE Y GPLVEEEEK VPVRPKDPEG VPPLLVSQQ A KREEERSP AA PPPLPTT SSG KAAKKP TAAE VSVRV PRG, corresponding to amino acids 1366-1468 of Human ALK, a region which spans the tyrosine kinase catalytic domain and part of the C-terminus of the NPM-ALK transcript.

  • 阳性对照
    • Anaplastic lymphoma
  • 常规说明The amino acid sequence used to raise this antibody was: aa 426-528.


    This antibody recognises a human p80 protein, identified as a hybrid of the anaplastic lymphoma kinase (ALK) gene and the nucleophosmin (NPM) gene resulting from the t(2;5)(p23;q35) translocation found in a third of large cell lymphomas. This rabbit polyclonal antibody can be used to detect p80 in these lymphomas and may also be used to detect a recently described subtype of large B cell lymphoma, which expresses the full-length ALK protein.

性能

应用

Our Abpromise guarantee covers the use of ab16670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 176 kDa. PubMed: 21847362
IHC-P 1/100.

Antigen retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.

靶标

  • 功能Neuronal receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK.
  • 组织特异性Expressed in brain and CNS. Also expressed in the small intestine and testis, but not in normal lymphoid cells.
  • 疾病相关A chromosomal aberration involving ALK is found in a form of non-Hodgkin lymphoma. Translocation t(2;5)(p23;q35) with NPM1. The resulting chimeric NPM1-ALK protein homodimerize and the kinase becomes constitutively activated. The constitutively active fusion proteins are responsible for 5-10% of non-Hodgkin lymphomas.
    A chromosomal aberration involving ALK is associated with inflammatory myofibroblastic tumors (IMTs). Translocation t(2;11)(p23;p15) with CARS; translocation t(2;4)(p23;q21) with SEC31A.
    A chromosomal aberration involving ALK is associated with anaplastic large-cell lymphoma (ALCL). Translocation t(2;17)(p23;q25) with ALO17.
    Neuroblastoma 3
    The ALK signaling pathway plays an important role in glioblastoma, the most common malignant brain tumor of adults and one of the most lethal cancers. It regulates both glioblastoma migration and growth.
    A chromosomal aberration involving ALK is found in one subject with colorectal cancer. Translocation t(2;2)(p23.1;p23.3). A 5 million base pair tandem duplication generates an in-frame WDCP-ALK gene fusion.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 1 LDL-receptor class A domain.
    Contains 2 MAM domains.
    Contains 1 protein kinase domain.
  • 翻译后修饰Phosphorylated at tyrosine residues by autocatalysis, which activates kinase activity. In cells not stimulated by a ligand, receptor protein tyrosine phosphatase beta and zeta complex (PTPRB/PTPRZ1) dephosphorylates ALK at the sites in ALK that are undergoing autophosphorylation through autoactivation. Phosphorylation at Tyr-1507 is critical for SHC1 association.
    N-glycosylated.
  • 细胞定位Cell membrane. Membrane attachment was crucial for promotion of neuron-like differentiation and cell proliferation arrest through specific activation of the MAP kinase pathway.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alk antibody
    • ALK tyrosine kinase receptor antibody
    • ALK/EML4 fusion gene, included antibody
    • ALK/NPM1 fusion gene, included antibody
    • ALK_HUMAN antibody
    • anaplastic lymphoma kinase (Ki-1) antibody
    • Anaplastic lymphoma kinase antibody
    • Anaplastic lymphoma kinase Ki1 antibody
    • anaplastic lymphoma receptor tyrosine kinase antibody
    • CD 246 antibody
    • CD246 antibody
    • CD246 antigen antibody
    • EC 2.7.10.1 antibody
    • Ki 1 antibody
    • Ki1 antibody
    • mutant anaplastic lymphoma kinase antibody
    • NBLST 3 antibody
    • NBLST3 antibody
    • Tcrz antibody
    • TFG/ALK antibody
    see all

Anti-ALK antibody [SP8] 图像

  • Immunohistochemical analysis of Human anaplastic lymphoma tissue labelling ALK with ab16670.

  • Immunohistochemical analysis of paraffin-embedded Human anaplastic lymphoma tissue labelling ALK with ab16670 at 1/100.

Anti-ALK antibody [SP8] (ab16670)参考文献

This product has been referenced in:
  • Wang YW  et al. Identification of oncogenic point mutations and hyperphosphorylation of anaplastic lymphoma kinase in lung cancer. Neoplasia 13:704-15 (2011). WB, IHC-P ; Human . Read more (PubMed: 21847362) »
  • Lagarrigue F  et al. Matrix metalloproteinase-9 is upregulated in nucleophosmin-anaplastic lymphoma kinase-positive anaplastic lymphomas and activated at the cell surface by the chaperone heat shock protein 90 to promote cell invasion. Cancer Res 70:6978-87 (2010). IHC-P ; Human . Read more (PubMed: 20699364) »

See all 3 Publications for this product

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Thank you for your enquiry and your interest in our products. The amino acid sequence used to raise this antibody was: aa 426-528. We hope this information will be useful for you.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"