概述

  • 产品名称Anti-ALIX抗体
    参阅全部 ALIX 一抗
  • 描述
    兔多克隆抗体to ALIX
  • 经测试应用适用于: IPmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan, Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Synthetic peptide corresponding to Human ALIX aa 400-450.

  • 阳性对照
    • HeLa whole cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab104256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at 2-5 µg/mg of lysate.
  • 应用说明Is unsuitable for WB.
  • 靶标

    • 功能Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Appears to be an adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. Involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function implies the interaction with CHMP4B. May play a role in the regulation of both apoptosis and cell proliferation.
    • 序列相似性Contains 1 BRO1 domain.
    • 细胞定位Cytoplasm > cytosol. Melanosome. Cytoplasm > cytoskeleton > centrosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Colocalized with CEP55 in the midbody during cytokinesis. Colocalized with CEP55 at centrosomes of non-dividing cells.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AIP1 antibody
      • ALG 2 interacting protein 1 antibody
      • ALG-2-interacting protein 1 antibody
      • ALG2 interacting protein X antibody
      • Alix antibody
      • Apoptosis linked gene 2 interacting protein X antibody
      • Dopamine receptor interacting protein 4 antibody
      • DRIP4 antibody
      • Hp95 antibody
      • KIAA1375 antibody
      • MGC17003 antibody
      • PDC6I_HUMAN antibody
      • PDCD6 interacting protein antibody
      • PDCD6-interacting protein antibody
      • PDCD6IP antibody
      • Programmed cell death 6 interacting protein antibody
      • Programmed cell death 6-interacting protein antibody
      see all

    Anti-ALIX antibody 图像

    • Detection of PDC6I in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab104256 at 3 µg/mg lysate for IP (Lane 1). For WB detection an anti-PDC6I antibody which recognizes a downstream epitope was used at 0.4 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.

    Anti-ALIX antibody (ab104256)参考文献

    ab104256 has not yet been referenced specifically in any publications.

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