Anti-ALIX抗体[3A9] (ab117600)


  • 产品名称Anti-ALIX抗体[3A9]
    参阅全部 ALIX 一抗
  • 描述
    小鼠单克隆抗体[3A9] to ALIX
  • 经测试应用适用于: WB, ELISA, IHC-P, ICC/IF, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Xenopus laevis, Xenopus tropicalis
  • 免疫原

    Fusion protein corresponding to Human ALIX.

  • 阳性对照
    • HeLa whole cell lysate



Our Abpromise guarantee covers the use of ab117600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 96 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Do not perform antigen retrieval.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.


  • 功能Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Appears to be an adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. Involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function implies the interaction with CHMP4B. May play a role in the regulation of both apoptosis and cell proliferation.
  • 序列相似性Contains 1 BRO1 domain.
  • 细胞定位Cytoplasm > cytosol. Melanosome. Cytoplasm > cytoskeleton > centrosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Colocalized with CEP55 in the midbody during cytokinesis. Colocalized with CEP55 at centrosomes of non-dividing cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AIP1 antibody
    • ALG 2 interacting protein 1 antibody
    • ALG-2-interacting protein 1 antibody
    • ALG2 interacting protein X antibody
    • Alix antibody
    • Apoptosis linked gene 2 interacting protein X antibody
    • Dopamine receptor interacting protein 4 antibody
    • DRIP4 antibody
    • Hp95 antibody
    • KIAA1375 antibody
    • MGC17003 antibody
    • PDC6I_HUMAN antibody
    • PDCD6 interacting protein antibody
    • PDCD6-interacting protein antibody
    • PDCD6IP antibody
    • Programmed cell death 6 interacting protein antibody
    • Programmed cell death 6-interacting protein antibody
    see all

Anti-ALIX antibody [3A9] 图像

  • Anti-ALIX antibody [3A9] (ab117600) + HeLa whole cell lysate

    Rabbit Anti Mouse IgG

    Predicted band size : 96 kDa

Anti-ALIX antibody [3A9] (ab117600)参考文献

This product has been referenced in:
  • Wiklander OP  et al. Extracellular vesicle in vivo biodistribution is determined by cell source, route of administration and targeting. J Extracell Vesicles 4:26316 (2015). Read more (PubMed: 25899407) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (Epethelial cells)
Specification Epethelial cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

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Verified customer

提交于 Aug 20 2014

Application Immunoprecipitation
Immuno-precipitation step Protein A/G
Sample Human Cell lysate - whole cell (Epethelial cells)
Specification Epethelial cells
Total protein in input 250 µg

Abcam user community

Verified customer

提交于 Aug 20 2014

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Here is the WB protocol you had requested.

Western Blotting

1. Blocking Buffer
5% non-fat dried milk dissolved in PBS.
Note: For the detection of phosphoproteins, NEVE...

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