KLH conjugated synthetic peptide corresponding to a region within N terminal amino acids 23-52 of Human ALG10.
A375 cell lysate; Human brain tissue
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Preservative: 0.09% Sodium Azide
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Immunogen affinity purified
ab103466 is purified through a protein A column, followed by peptide affinity purification.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/100 - 1/500. Predicted molecular weight: 56 kDa.
ALG10 encodes a membrane-associated protein that adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. That is, it transfers the terminal glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc2Man9GlcNAc(2)-PP-Dol. The rat protein homolog was shown to specifically modulate the gating function of the rat neuronal ether-a-go-go(EAG) potassium ion channel.
Endoplasmic reticulum membrane; Multi-pass membrane protein
ALG 10 antibody
Alpha 1,2 glucosyltransferase ALG10 A antibody
Western blot - ALG10 antibody (ab103466)
Anti-ALG10 antibody (ab103466) at 1/100 dilution + A375 cell lysate at 35 µg
Predicted band size : 56 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ALG10 antibody (ab103466)
ab103466, at 1/50 dilution, staining ALG10 in formalin-fixed, paraffin-embedded Human brain tissue, using a peroxidase conjugated secondary antibody and DAB staining.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"