Aldosterone ELISA试剂盒(ab136933)
Key features and details
- Sensitivity: 4.7 pg/ml
- Range: 3.9 pg/ml - 250 pg/ml
- Sample type: Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Mouse, Rat, Human, Mammals
概述
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产品名称
Aldosterone ELISA试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Aldosterone 20 84.8pg/ml 4.5% Aldosterone 20 27.7pg/ml 4.4% Aldosterone 20 13.9pg/ml 6.6% 批次间 样品 n Mean SD CV% Aldosterone 13 72.6pg/ml 10.8% Aldosterone 13 24.4pg/ml 18% Aldosterone 13 10.8pg/ml 16.3% -
样品类型
Urine, Serum, Plasma -
检测类型
Competitive -
灵敏度
= 4.7 pg/ml -
范围
3.9 pg/ml - 250 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Urine = 98.7 7.5pg/ml - 120pg/ml Serum = 69.8 7.5pg/ml - 120pg/ml Plasma = 108.3 7.5pg/ml - 120pg/ml -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse, Rat, Human, Mammals -
产品概述
Abcam’s Aldosterone in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Aldosterone in plasma, serum and urine.
A donkey anti-sheep IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated- Aldosterone antigen and a polyclonal sheep antibody specific to Aldosterone. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Aldosterone captured in the plate.
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说明
Compound % Cross Reactivity 11-Deoxycorticosterone
0.30%
Progesterone
0.20%
Corticosterone 0.19%
Cortisol ≤ 0.001%
DHT ≤ 0.001%
Estradiol ≤ 0.001%
Testosterone ≤ 0.001%
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer Concentrate 1 x 27ml Aldosterone Alkaline Phosphatase Conjugate 1 x 5ml Aldosterone Antibody 1 x 5ml Aldosterone Standard 1 x 250µl Assay Buffer 1 x 27ml Donkey anti-sheep IgG Microplate (12 x 8 wells) 1 unit Plate Sealer 1 unit pNpp Substrate 1 x 20ml Stop Solution 1 x 5ml -
研究领域
图片
数据表及文件
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SDS download
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Datasheet download
文献 (28)
ab136933 被引用在 28 文献中.
- Okamoto K et al. Changes of FGF23 and the Renin-Angiotensin-System in Male Mouse Models of Chronic Kidney Disease and Cardiac Hypertrophy. J Endocr Soc 6:bvab187 (2022). PubMed: 35047715
- Schreckenberg R et al. Effect of Anti-Hypertensive Medication on Plasma Concentrations of Lysyl Oxidase: Evidence for Aldosterone-IL-6-Dependent Regulation of Lysyl Oxidase Blood Concentration. Biomedicines 10:N/A (2022). PubMed: 35885053
- Yan F et al. Combination of tolvaptan and valsartan improves cardiac and renal functions in doxorubicin-induced heart failure in mice. Eur J Histochem 66:N/A (2022). PubMed: 36373350
- Shaw J et al. Effectiveness of short-term isothermic-heat acclimation (4 days) on physical performance in moderately trained males. PLoS One 17:e0270093 (2022). PubMed: 36395344
- Abou Nader N et al. Effect of Inactivation of Mst1 and Mst2 in the Mouse Adrenal Cortex. J Endocr Soc 7:bvac143 (2022). PubMed: 36405866