Anti-Aldolase B抗体(ab153828)
Key features and details
- Rabbit polyclonal to Aldolase B
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Aldolase B抗体
参阅全部 Aldolase B 一抗 -
描述
兔多克隆抗体to Aldolase B -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to Human Aldolase B aa 15-265.
Database link: P05062 -
阳性对照
- Recombinant Human Aldolase B protein (ab123165) can be used as a positive control in WB. 293T, A431, HeLa, Neuro2A, GL261, C8D30 and PC12 whole cell lysates; Hela cells; Human hepatoma tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab153828于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 39 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 39 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
靶标
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通路
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4. -
疾病相关
Defects in ALDOB are the cause of hereditary fructose intolerance (HFI) [MIM:229600]. HFI is an autosomal recessive disease that results in an inability to metabolize fructose and related sugars. Complete exclusion of fructose results in dramatic recovery; however, if not treated properly, HFI subjects suffer episodes of hypoglycemia, general ill condition, and risk of death the remainder of life. -
序列相似性
Belongs to the class I fructose-bisphosphate aldolase family. - Information by UniProt
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数据库链接
- Entrez Gene: 229 Human
- Entrez Gene: 230163 Mouse
- Entrez Gene: 24190 Rat
- Omim: 612724 Human
- SwissProt: P05062 Human
- SwissProt: Q91Y97 Mouse
- SwissProt: P00884 Rat
- Unigene: 530274 Human
see all -
别名
- ALDB antibody
- ALDO B antibody
- ALDO2 antibody
see all
图片
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All lanes : Anti-Aldolase B antibody (ab153828) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 39 kDa
10% SDS PAGE -
Anti-Aldolase B antibody (ab153828) at 1/1000 dilution + PC12 whole cell lysate at 30 µg
Predicted band size: 39 kDa
10 % SDS-PAGE -
All lanes : Anti-Aldolase B antibody (ab153828) at 1/1000 dilution
Lane 1 : Neuro2A whole cell lysate
Lane 2 : GL261 whole cell lysate
Lane 3 : C8D30 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 39 kDa
10 % SDS-PAGE -
Immunofluorescence analysis of methanol fixed HeLa cells labeling Aldolase B with ab153828 at 1/500. Lower panel shows costaining with Hoechst 33342.
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Immunohistochemical analysis of paraffin embedded Human hepatoma tissue labeling ALDOB with ab153828 at 1/500.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab153828 被引用在 2 文献中.
- Jeong S et al. High Fructose Drives the Serine Synthesis Pathway in Acute Myeloid Leukemic Cells. Cell Metab 33:145-159.e6 (2021). PubMed: 33357456
- Lian J et al. Aldolase B impairs DNA mismatch repair and induces apoptosis in colon adenocarcinoma. Pathol Res Pract 215:152597 (2019). PubMed: 31564566