The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/50.
1/50 - 1/100. Detects a band of approximately 36 kDa (predicted molecular weight: 39 kDa).
Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
Defects in ALDOA are the cause of glycogen storage disease type 12 (GSD12) [MIM:611881]; also known as red cell aldolase deficiency. A metabolic disorder associated with increased hepatic glycogen and hemolytic anemia. It may lead to myopathy with exercise intolerance and rhabdomyolysis.
Belongs to the class I fructose-bisphosphate aldolase family.
ab71433 at 1/50 dilution staining Aldolase - N - terminal in human lung carcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.