概述

  • 产品名称Anti-Aldolase抗体
    参阅全部 Aldolase 一抗
  • 描述
    小鼠单克隆抗体to Aldolase
  • 经测试应用适用于: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-365 of Human Aldolase

  • 阳性对照
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human skeletal muscle.

性能

应用

Our Abpromise guarantee covers the use of ab54770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 39 kDa.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.
IP Use a concentration of 5 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.
  • 通路Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
  • 疾病相关Defects in ALDOA are the cause of glycogen storage disease type 12 (GSD12) [MIM:611881]; also known as red cell aldolase deficiency. A metabolic disorder associated with increased hepatic glycogen and hemolytic anemia. It may lead to myopathy with exercise intolerance and rhabdomyolysis.
  • 序列相似性Belongs to the class I fructose-bisphosphate aldolase family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ALDA antibody
    • Aldo1 antibody
    • ALDOA antibody
    • ALDOA_HUMAN antibody
    • Aldolase 1 antibody
    • Aldolase A antibody
    • Aldolase A fructose bisphosphatase antibody
    • Aldolase A fructose bisphosphate antibody
    • FRUCTOALDOLASE A antibody
    • Fructose 1 6 bisphosphate triosephosphate lyase antibody
    • Fructose bisphosphate aldolase A antibody
    • Fructose bisphosphate aldolase antibody
    • FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE A antibody
    • Fructose-bisphosphate aldolase A antibody
    • Fructose-bisphosphate aldolase A Muscle-type antibody
    • GSD12 antibody
    • Lung cancer antigen NY LU 1 antibody
    • Lung cancer antigen NY-LU-1 antibody
    • MGC10942 antibody
    • MGC17716 antibody
    • MGC17767 antibody
    • Muscle type aldolase antibody
    • Muscle-type aldolase antibody
    • RNALDOG5 antibody
    see all

Anti-Aldolase antibody 图像

  • Aldolase was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Mouse monoclonal to Aldolase and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab54770.

    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.

    Band: 39kDa; Aldolase



  • Predicted band size : 39 kDa
    Aldolase antibody (ab54770) at 1ug/lane + A-431 cell lysate at 25ug/lane.
  • ab54770 at 10 ug/ml staining Aldolase in human Hela cells by Immunocytochemistry / Immunofluorescence.

  • IHC image of Aldolase staining in Human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab54770, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Overlay histogram showing HepG2 cells stained with ab54770 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54770, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Aldolase antibody (ab54770)参考文献

This product has been referenced in:
  • Sørensen BS  et al. Proteins upregulated by mild and severe hypoxia in squamous cell carcinomas in vitro identified by proteomics. Radiother Oncol 92:443-9 (2009). WB ; Human . Read more (PubMed: 19541378) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (A549 Lung cancer cells)
Specification A549 Lung cancer cells
Permeabilization Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative Paraformaldehyde
Username

Dr. Dimitra Kalamida

Verified customer

提交于 Feb 17 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HCT116)
Loading amount 10 µg
Specification HCT116
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Oct 13 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human frontal cortex)
Specification human frontal cortex
Fixative Formaldehyde
Permeabilization Yes - heat-mediated TRIS/EDTA buffer pH9
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 May 19 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Huh 7.5 cells)
Loading amount 12 µg
Specification Huh 7.5 cells
Gel Running Conditions Reduced Denaturing (12.5% gel)
Blocking step casein as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 27 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"