概述

  • 产品名称
    Anti-ALDH9A1抗体
    参阅全部 ALDH9A1 一抗
  • 描述
    兔多克隆抗体to ALDH9A1
  • 经测试应用
    适用于: WB, Indirect ELISA, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide (Human)from the C-terminal region of human ALDH9A1 conjugated to KLH.

  • 阳性对照
    • Mouse kidney tissue lysates

性能

应用

Our Abpromise guarantee covers the use of ab79726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50 - 1/100. Predicted molecular weight: 54 kDa.
Indirect ELISA 1/1000.
ELISA Use at an assay dependent concentration.

靶标

  • 功能
    Converts gamma-trimethylaminobutyraldehyde into gamma-butyrobetaine. Catalyzes the irreversible oxidation of a broad range of aldehydes to the corresponding acids in an NAD-dependent reaction.
  • 组织特异性
    High expression in adult liver, skeletal muscle, and kidney. Low levels in heart, pancreas, lung and brain. Expressed in all regions of the brain. Expression levels are variable in the different brain areas, with the highest levels in the spinal cord and the lowest in the occipital pole.
  • 通路
    Amine and polyamine biosynthesis; carnitine biosynthesis.
  • 序列相似性
    Belongs to the aldehyde dehydrogenase family.
  • 发展阶段
    Strongly expressed in human embryonic brain (gestational age 12 weeks).
  • 翻译后修饰
    The N-terminus is blocked.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 4 trimethylaminobutyraldehyde dehydrogenase antibody
    • 4-trimethylaminobutyraldehyde dehydrogenase antibody
    • AL9A1_HUMAN antibody
    • aldehyde dehydrogenase (NAD+) antibody
    • Aldehyde dehydrogenase 9A1 antibody
    • Aldehyde dehydrogenase E3 isozyme antibody
    • Aldehyde dehydrogenase family 9 member A1 antibody
    • ALDH4 antibody
    • ALDH7 antibody
    • ALDH9 antibody
    • aldh9A1 antibody
    • E3 antibody
    • Gamma aminobutyraldehyde dehydrogenase antibody
    • Gamma-aminobutyraldehyde dehydrogenase antibody
    • R aminobutyraldehyde dehydrogenase antibody
    • R-aminobutyraldehyde dehydrogenase antibody
    • TMABADH antibody
    see all

图片

  • Anti-ALDH9A1 antibody (ab79726) at 1/50 dilution + mouse kidney tissue lysate at 35 µg

    Predicted band size : 54 kDa
    Observed band size : 54 kDa

实验方案

文献

This product has been referenced in:

See 1 Publication for this product

客户评价及客户问答

Application
Immunoprecipitation
Sample
Human Tissue lysate - whole (brain)
Total protein in input
500 µg
Specification
brain
Immuno-precipitation step
Other - dynabead
Username

Abcam user community

Verified customer

提交于 May 04 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (brain)
Specification
brain
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% Triton x-100
Blocking step
FBS as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 May 04 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (BRAIN)
Loading amount
50 µg
Specification
BRAIN
Gel Running Conditions
Non-reduced Denaturing (12%PA GEL)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Apr 09 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Human Serum (blood)
Specification
blood
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: RT°C
Type
Direct
Username

Abcam user community

Verified customer

提交于 Apr 09 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (neuronal cell line HpL3-4)
Loading amount
30 µg
Specification
neuronal cell line HpL3-4
Gel Running Conditions
Non-reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C
Username

Abcam user community

Verified customer

提交于 Oct 06 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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